QuEChERS-气相色谱串联质谱法同时测定番茄中46种农药残留

建立一种QuEChERS结合气相色谱-串联质谱同时检测番茄中46种杀菌剂和杀虫剂残留的分析方法。样品用乙腈提取，经PSA、C₁₈、无水MgSO₄净化，HP-5MS色谱柱分离，采用多反映监测（MRM）模式检测，基质匹配外标法定量。结果表明，46种农药在0.005～0.500 μg/mL的浓度范围内线性关系良好（R2>0.999），方法检出限和定量限分别在0.07～2.63 μg/kg和0.21～8.39 μg/kg之间；在0.0025、0.025、0.25 mg/kg的加标水平下，46种农药化合物平均加标回收率在70.40%～115.08%范围内，相对标准偏差为0.22%～4.89%。50批次番茄样品中9批次检出毒死蜱和嘧菌酯残留，含量范围在0.0043～0.0365 mg/kg，均未超出GB 2763-2021标准规定的限量值。本方法快速、简单、灵敏、准确，能够满足番茄中多农药残留的检测。

Simultaneous Determination of 46 Pesticide Residues in Tomatoes by QuEChERS Method Combined with Gas Chromatography-Mass Spectrometry

An analysis method for simultaneous determination of 46 kinds of fungicides and insecticides residues in tomatoes by QuEChERS method combined with gas chromatography-mass spectrometry was established. The samples were extracted with acetonitrile, then purified up with PSA, C₁₈ and anhydrous MgSO₄, separated by a HP-5MS chromatographic column, analyzed using multiple reflection monitoring (MRM) mode, and quantified by matrix-matched external standard method. The result showed that, the 46 kinds of pesticides showed good linearity when they were in 0.005～0.500 μg/mL (R2>0.999). The limits of detection of the 46 kinds of pesticides were in 0.07～2.63 μg/kg, and the limits of quantitation were in 0.21～8.39 μg/kg. The average recoveries were 70.40%～115.08% with the relative standard deviations of 0.22%～4.89% at the spiked levels of 0.0025, 0.025 and 0.25 mg/kg. Residues of chlorpyrifos and azoxystrobin were detected in 9 of the 50 batches of tomato samples, with the content ranging from 0.0043～0.0365 mg/kg, which did not exceed the limit value specified in GB 2763-2021. The method was rapid, simple, sensitive and accurate, which could meet the detection of multiple pesticide residues in tomato.