基于体外模拟消化的黑脉羊肚菌多酚细胞抗氧化及抗增殖活性

以黑脉羊肚菌多酚提取物为研究对象，通过体外模拟消化实验，研究消化过程中消化液多酚含量及氧自由基吸收能力的变化；研究体外模拟胃、肠消化对人肝癌细胞（HepG2细胞）和人结肠癌细胞（Caco-2细胞）的毒性及抗增殖活性的影响；并以HepG2细胞为模型对细胞抗氧化活性（cellular antioxidant activity，CAA）进行评价。结果表明：体外模拟消化后，黑脉羊肚菌多酚释放及氧自由基吸收能力均有提高，模拟胃、肠消化过程中多酚释放分别在1 h和0.5 h达到最高并保持稳定。细胞实验表明，在不产生细胞毒性的范围内，胃0 h、胃液1 h、肠液0.5 h对HepG2的抑制率分别为41%、45%、91%；对Caco-2细胞的抑制率分别为73%、96%、90%。CAA结果显示，胃消化处理的样品表现出促氧化的作用，肠消化处理的样品表现出抗氧化的作用，肠液0.5 h的CAA值为（70.506±0.011）μmol QE/100 g（以干质量计）。由此可见，体外模拟消化处理黑脉羊肚菌具有一定的抗氧化及抗增殖活性，为羊肚菌功能活性的开发提供了理论依据。

Antioxidant and Antiproliferative Activity in Human Cancer Cells of Polyphenols Extracted from Morchella angusticepes Peck Based on in Vitro Simulated Digestion

Morchella angusticepes Peck was subjected to in vitro simulated gastrointestinal digestion. Changes in
polyphenol content and antioxidant activity in terms of oxygen radical absorption capacity (ORAC) were determined during
the digestion process. The anti-proliferative activity and cytotoxicity of digests were measured in HepG2 cells and Caco-2
cells as a function of digestion time. Antioxidant activity was evaluated as well by cellular antioxidant activity (CAA) assay
in HepG2 cells. The results showed that after the gastrointestinal digestion, polyphenol content and ORAC were increased
significantly. Polyphenol release reached the maximum level and then remained stable after 1 h gastric digestion and 0.5 h
intestinal digestion, respectively. The percentage inhibition of HepG2 cells by Morchella angusticepes Peck and its 1 h
gastric and 0.5 h intestinal digests were 41%, 45% and 91%, respectively, and the percentage inhibition of Caco-2 cells
were 73%, 96% and 90%, respectively. The gastric digests showed pro-oxidant effect, whereas the intestinal digests showed
antioxidant capacity. The CAA value of the 0.5 h intestinal digest was (70.506 ± 0.011) μmol QE/100 g md. In conclusion,
in vitro digestion of Morchella angusticepes Peck showed anti-proliferation and cellular antioxidant activity, which could
provide a theoretical basis for further development and utilization of Morchella angusticepes Peck.