| 表面等离子共振传感检测Cry2A蛋白 |
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| 引用本文： | 蔡 淼,黄 新,岳喜庆.表面等离子共振传感检测Cry2A蛋白[J].食品科学,2014,35(24):201-204. |
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| 作者姓名： | 蔡 淼 黄 新 岳喜庆 |
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| 作者单位： | 1.沈阳农业大学食品学院，辽宁 沈阳 110866；2.中国检验检疫科学研究院植物检疫研究所，北京 100029 |
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| 基金项目： | 质检公益性行业科研专项（201410014） |
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| 摘 要： | 目的：建立利用表面等离子共振（surface plasmon resonance，SPR）传感器检测转基因植物中苏云金芽孢杆菌Cry2A蛋白的方法。方法：利用SPR检测技术，根据生物分子间的相互作用，在金片表面修饰Cry2A蛋白的特异性单克隆抗体，对不同质量浓度梯度的Cry2A蛋白进行检测研究。结果：该方法可有效地检测到Cry2A蛋白，检测灵敏度可达10 ng/mL，与同为抗虫基因编码的Cry1Ac和Cry2Ab蛋白未出现交叉反应。结论：利用SPR方法检测Cry2A蛋白操作简单，省时且灵敏度高、特异性强，可用于对Cry2A蛋白的定性检测。
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| 关 键 词： | 表面等离子共振传感器 Cry2A蛋白 苏云金芽孢杆菌 转基因植物 |
Using a Surface Plasmon Resonance Sensor for the Detection of Bacillus thuringiensis Cry2A Protein |
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| CAI Miao,HUANG Xin,YUE Xi-qing.Using a Surface Plasmon Resonance Sensor for the Detection of Bacillus thuringiensis Cry2A Protein[J].Food Science,2014,35(24):201-204. |
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| Authors: | CAI Miao HUANG Xin YUE Xi-qing |
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| Affiliation: | 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China;
2. Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, China |
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| Abstract: | Purpose: To establish a method to detect Bacillus thuringiensis (Bt) Cry2A protein by surface plasmon resonance
(SPR) sensor. Methods: By using SPR based on interaction between biomolecules, the monoclonal antibodies specific to
the Cry2A protein were modified on the gold surface for the detection of this protein. Results: The detection limit of Cry2A
protein was 10 ng/mL by this method without cross-reaction with Cry1Ac or Cry2Ab protein. Conclusion: The SPR method
was simple, time-saving, sensitive, specific and applicable for the qualitative detection of Bt Cry2A protein. |
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| Keywords: | surface plasmon resonance sensor Cry2A protein Bacillus thuringiensis genetically modified plant |
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