QuEChERS-超高效液相色谱-串联质谱法测定食品中5种罂粟壳生物碱基质效应

目的考察3种不同食品基质中罂粟碱、吗啡等5种生物碱类物质在超高效液相色谱-串联质谱法检测中的基质效应。方法用提取前与提取后空白溶液加标方法分析基质效应的影响,并配制不同浓度加标溶液,通过拟合标准曲线,比较斜率、截距等,评估5种生物碱成分测定的基质效应。结果固体、半固体基质样品基质效应较强,那可丁、蒂巴因、可待因易受基质效应影响,通过优化梯度洗脱程序、加入内标溶液、稀释样品等方法可有效降低或消除基质效应影响。结论基质效应与基质种类、化合物种类等有明显关系,本试验中基质效应的改进方法可为不同样品基质中罂粟壳生物碱的测定提供参考。

Matrix effects in the analysis of five poppy husk alkaloids in food by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry

Objective To investigate the matrix effects of 5 alkaloids such as papaverine and morphine in 3 different food matrices by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry. Methods The matrix effect was analyzed before and after the extraction of the blank solution spiked with different concentration of the standard solution. The matrix effect of the determination of the 5 alkaloids was evaluated by fitting the standard curve and comparing the slope and intercept. Results The matrix effect of solid and semisolid matrix were strong, and narcotine, thebaine and codeine were easily influenced by the matrix effect. The effect of matrix effect could be effectively reduced or eliminated by optimizing the gradient elution program, adding internal standard solution and dilution samples. Conclusion The matrix effect was closely related to the type of sample matrix and the structure of compound. The improved method of matrix effect in this study could provide reference for the determination of poppy husk alkaloids in different sample matrix.