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| 酿酒酵母纤维二糖代谢途径的搭建 | |
| 引用本文： | 张梁,石贵阳,洪剑辉,王正祥,章克昌. 酿酒酵母纤维二糖代谢途径的搭建[J]. 酿酒科技, 2005, 0(3): 30-33 |
| 作者姓名： | 张梁 石贵阳 洪剑辉 王正祥 章克昌 |
| 作者单位： | 1. 江南大学工业生物技术教育部重点实验室,江苏,无锡,214036;江南大学生物工程学院生物资源研究室,江苏,无锡,214036 2. 江南大学生物工程学院生物资源研究室,江苏,无锡,214036 3. 江南大学工业生物技术教育部重点实验室,江苏,无锡,214036 |
| 基金项目： | 国家科技部“十五”攻关项目(2001BA501A01)资助 |
| 摘 要： | 酒糟中存在着一些未能被酵母消耗的糖，含量较高的如纤维二糖、蜜二糖等，有效利用酒精发酵过程中产生的纤维二糖，具有一定理论和实际意义。采用异硫氰酸胍一酚一氯仿法提取里氏木霉总RNA，分离poly A^ mRNA，通过RT-PCR方法扩增得到β-葡萄糖苷酶基因。构建了重组质粒pYX-BGL，并在酿酒酵母Saccharomyces cerevisiae W303-1A中获得表达，得到的转化子能以纤维二糖为唯一碳源生长。 |
| 关 键 词： | 微生物 酿酒酵母 β-葡萄糖苷酶 纤维二糖利用 |
| 文章编号： | 1001-9286(2005)03-0030-04 |
| 修稿时间： | 2004-12-08 |
Metabolism Pathway of Cellobiose in Saccharomyces cerevisiae |
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| ZHANG Liang,SHI Gui-yang,HONG Jian-hui,WANG Zheng-xiang,ZHANG Ke-chang. Metabolism Pathway of Cellobiose in Saccharomyces cerevisiae[J]. Liquor-making Science & Technology, 2005, 0(3): 30-33 | |
| Authors: | ZHANG Liang SHI Gui-yang HONG Jian-hui WANG Zheng-xiang ZHANG Ke-chang |
| Affiliation: | ZHANG Liang1,2,SHI Gui-yang1,2,HONG Jian-hui2,WANG Zheng-xiang1,ZHANG Ke-chang2 |
| Abstract: | There was some unconsumed sugar in distiller's grains such as cellobiose and melibiose etc. Effective utilization of cellobiose produced during alcohol fermentation was of theoretical and practical significance. The total RNA of Trichoderma reesei was extracted by guanidine thiocyanate-phenyl-chloroform extraction method, from which the poly A mRNA was purified. The gene encoding purified mRNA as template. The recombined plasmid pYX-BGL was constructed and expressed in Saccharomyces cerevisiae W303-1A. The recombinants can grow well on cellubiose as a sole carbon source. (Tran. by YUE Yang) |
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