贻贝蒸煮液体外抗氧化及阻断N-亚硝胺合成的研究

为了研究贻贝蒸煮液的抗氧化活性和阻断亚硝胺合成能力,采用DPPH.法测定了试样的抗氧化活性,并模拟胃液在体外进行亚硝胺合成阻断实验,测定了试样对亚硝酸根离子的清除率和N-亚硝胺合成的阻断率。结果表明,在DPPH.清除实验中,贻贝蒸煮液(以可溶性固形物计)的IC50为3.80μg/mL,对照品TBHQ的IC50为7.55μg/mL。另外,贻贝蒸煮液清除亚硝酸根离子与阻断N-亚硝胺合成的最佳剂量水平(以可溶性固形物计)为64μg/mL,此时,清除率和阻断率分别达到84.08%和71.04%,相同浓度下,对照品VC的清除率和阻断率分别为30.90%和29.27%。

Study on the anti-oxidation activity and blocking synthesis of nitrosamine of mussel juice in vitro

To investigate the capabilities of mussel juice (MJ) on anti-oxidation activity and blocking synthesis of NDMA, the method of DPPH were applied to measure the anti-oxidation activity of every sample and the process external how the human gastric juice blocks synthesis of NDMA were simulated, and the rate of blocking NDMA synthesis and the rate of scavenging sodium nitrite for every sample were determined.The results showed that for the experiment of scavenging of DPPH·,the content of IC_(50)in MJ equaled to 3.8μg/mL that was measured by soluble solid,while the content of IC_(50) in TBHQ equaled to 7.55μg/mL.In addition,64μg/mL was found to be the best concentration of soluble solid for MJ to scavenge NaNO_2 and block synthesis of NDMA.In this concentration, the rate of scavenging and blocking of MJ was 84.08% and 71.04% while those control article V_C were 30.90% and 29.27% respectively.