正交分析法优化裂褶菌多糖的微波辅助提取工艺

采用响应曲面法优选和探讨微波提取裂褶菌多糖的最优工艺。在单因素试验的基础上采用SAS 8.2软件设计试验,用响应面分析优化提取时间、料液比、提取温度、微波强度各因素及其相互作用的最佳组合。结果表明,裂褶菌多糖的最优提取工艺条件提取时间为20 min,料液比为1∶28.5,提取温度为51℃,微波强度为548 W,此条件下,多糖实际提取率为1.765%。与其他方法比较,微波提取方法时间短,得率高,是裂褶菌多糖提取的一种优选方法。

Optimization of microwave extraction of schizophyllan by using response surface method

Objective：To obtain optimal extraction parameters of Schizophyllan under microwave irradiation. We investigated extraction time,ratio of solvent to raw material, extraction temperature and microwave power on polysaccharides yield using single-factor experiment. Then we optimized extraction condition of Schizophyl-lan by using SAS 8 . 2 . Results showed that obtained optimal extraction of Schizophyllan is as follows：extraction time is 20 min,ratio of solvent to raw material is 28. 5, extraction temperature is 51℃, microwave power is 548 W. Under the optimal condition,yield of Schizophyllan is 1. 765%. Conclusion： In contrast to other ex-traction methods, the extraction rate of mocrowave technique is better and the time is shorter. The microwave technique is a better method for extracting Schizophyllan.