两种微波装置提取柑橘皮总黄酮的工艺比较

分别利用微波化学反应器(简称微化装置)和微波消解/萃取系统(简称微消装置)提取柑橘皮总黄酮。通过L9(34)正交实验设计,以固液比、乙醇浓度、提取时间、微波功率(微化装置)或提取温度(微消装置)为考察因素,分别优化并比较了2种微波装置的提取工艺。微化装置最佳提取工艺条件为:固液比1:30,70%乙醇作提取剂,于240W下微波提取2.0min,此时总黄酮得率为4.72mg/g;微消装置最佳工艺条件为:固液比1:20,70%乙醇作提取剂,于160℃下微波萃取3.0min,此时总黄酮得率为9.15mg/g,得率约为前者的2倍。分析讨论了微消装置得率较高的原因,分别应用2种微波装置提取3个不同品种的柑橘皮样品。结果表明:微消装置提取柑橘皮总黄酮较常规微波辅助提取法更为有效,可用于高效、快速、简便地提取天然产物有效成分。

Comparison of extraction technology of total flavonoids from citrus peels by two microwave extraction systems

The microwave chemical reaction system and microwave digested/extraction system for total flavonoids(TF) from citrus peels were optimized and compared.The solvent quantum to citrus peel(g:mL),ethanol content(%),extraction time(min),microwave power(W)(in microwave chemical reaction system) or extraction temperature(℃)(in microwave digested/extraction system) were reviewed using the L9(34) orthogonal design.The extraction conditions of the microwave extraction methods were both optimized.The optimum ethanol extraction process conditions were as follows:adding 30 fold of 70% ethanol,extracting for 2.0 min under 240 W power in microwave chemical reaction system,the extraction yield was 4.72 mg/g;adding 20 fold of 70% ethanol,extracting for 3.0 min at 160 ℃ in microwave digested/extraction system with extraction yield 9.15 mg/g.As a result,the extraction yield for TF from citrus peels was almost two times than the former.The reason was discussed.Three different samples of citrus peels were determined and compared with the optimal extraction conditions using these two microwave methods,respectively.The microwave digested/extraction system can be used in rapidly and simply extracting active components from natural products,for it has higher extraction yield than the general microwave-assisted extraction.