浸提法提取、双水相体系纯化红花红色素及稳定性研究

对红花进行预处理后,用一定浓度的丙酮溶液提取红花红色素,将提取液加入无机盐形成双水相对红色素进行纯化。研究讨论了丙酮的浓度、提取时间、料液比对提取率的影响,并对产品稳定性进行试验。实验结果表明红色素的提取工艺为:50%的丙酮料液比1g:10mL室温下超声辅助提取60min,采用丙酮-硫酸铵双水相体系对红色素进行纯化2次,将上相中的丙酮挥发、离心得纯化产品,提取率为84.9%。稳定性试验结果表明,红花红色素对温度、光和部分金属离子敏感。

Extracting carthamine from carthamus tinctorius by acetone aqueous and purifying by two-phase system

Carthamine from carthamus tinctorius pretreated was extracted by aqueous acetone.Acetoneammonium sulfate aqueous two-phase system was used for purifying carthamin.The main factors on the extraction technology were discussed,such as acetone concentration,extraction time and power to liquid ratio.The result were as follows:aqueous acetone 50%,solid to liquid ratio 1 g:10 mL,Ultrasonicextraction time 60 min,acetone-ammonium sulfate aqueous two-phase system was purified carthamin two times.Under the conditions,the yield was 84.9%.Stability test showed that carthamin was sensitized to temperature,light and metal ions.