| RP-HPLC法测定石榴汁中鞣花酸的含量 |
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| 引用本文： | 彭海燕,陈祥贵,刘振平,何宇新,杨文宇,杨潇,李玉锋.RP-HPLC法测定石榴汁中鞣花酸的含量[J].食品科技,2012(4):283-285. |
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| 作者姓名： | 彭海燕 陈祥贵 刘振平 何宇新 杨文宇 杨潇 李玉锋 |
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| 作者单位： | 西华大学生物工程学院食品生物技术四川省高校重点实验室 |
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| 基金项目： | 国家星火计划项目(2010GA810002);四川省科技攻关项目(05SG011-021) |
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| 摘 要： | 目的:建立检测石榴汁中鞣花酸的含量测定方法。方法;采用高效液相色谱法,ArcusEP-C18(250mm×4.6mm,5μm)色谱柱,以甲醇-0.1%TFA为流动相梯度洗脱,流速1.0mL/min,检测波长254nm。结果:鞣花酸0.004～0.064μg之间线性关系良好,平均加样回收率为99.32%。结论:方法精密可靠,可作为检测石榴汁中鞣花酸含量的方法。
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| 关 键 词： | 石榴汁 鞣花酸 反相高效液相色谱法 |
Determination of ellagic acid in the pomegranate juice by RP-HPLC |
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| PENG Hai-yan,CHEN Xiang-gui,LIU Zhen-ping,HE Yu-xin,YANG Wen-yu,YANG Xiao,LI Yu-feng.Determination of ellagic acid in the pomegranate juice by RP-HPLC[J].Food Science and Technology,2012(4):283-285. |
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| Authors: | PENG Hai-yan CHEN Xiang-gui LIU Zhen-ping HE Yu-xin YANG Wen-yu YANG Xiao LI Yu-feng |
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| Affiliation: | (School of Bioengineering Key Laboratory of Food Biotechnology of Sichuan Provincial University,Xihua University,Chengdu 610039) |
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| Abstract: | Objective:To establish HPLC method for determination of ellagic acid in the pomegranate juice.Method:The RP-HPLC was carried out on Arcus EP-C 18(250 mm×4.6 mm,5 μm).The gradient elution was performed by a mobile phase consisted a mixture of methanol and 0.1% TFA at a flow rate of 1.0 mL/min,and detection wavelength was at 254 nm.Results:The linear range was 0.004～0.064 μg for ellagic acid.The average recoveries was 99.32%.Conclusion:The method is accurate and reproducible for quality determination of ellagic acid in pomegranate juice. |
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| Keywords: | pomegranate juice ellagic acid RP-HPLC |
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