| 鼠李糖乳杆菌发酵液中水溶蛋白的提取及其抗氧化活性研究 |
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| 引用本文： | 张静,王丹,张晶,王玉华. 鼠李糖乳杆菌发酵液中水溶蛋白的提取及其抗氧化活性研究[J]. 食品科技, 2012, 0(8): 235-239 |
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| 作者姓名： | 张静 王丹 张晶 王玉华 |
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| 作者单位： | 吉林农业大学食品科学与工程学院 |
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| 基金项目： | 国家青年科学基金项目(31101252);吉林省科技引导计划项目(20090576) |
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| 摘 要： | 采用硫酸铵沉淀法获得鼠李糖乳杆菌B10发酵液中水溶蛋白粗提物,并进行葡聚糖凝胶SephadexG-100柱纯化,分离得到不同的蛋白组分峰Ⅰ、峰Ⅱ和峰Ⅲ,分别对各组分DPPH自由基清除能力、清除超氧自由基(O2-·)和羟自由基(·OH)的能力及超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性进行分析。结果表明,纯化前后各样品均对DPPH·有清除作用,其中纯化后的峰Ⅰ对DPPH·清除能力最强,峰Ⅱ、峰Ⅲ次之,均高于水溶蛋白粗提物;纯化后的峰Ⅰ对O2-·和·OH的清除能力最强,均高于5mmol/L的Vc溶液和水溶蛋白粗提物对O2-·和·OH的清除作用,峰Ⅱ和峰Ⅲ均未检测到对O-·和·OH的清除能力。
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| 关 键 词： | 鼠李糖乳杆菌 水溶蛋白 抗氧化 |
Extraction and antioxidant activity of the soluble protein from Lactobacillus rhamnosus broth |
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| ZHANG Jing, WANG Dan, ZHANG Jing, WANG Yu-hua. Extraction and antioxidant activity of the soluble protein from Lactobacillus rhamnosus broth[J]. Food Science and Technology, 2012, 0(8): 235-239 |
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| Authors: | ZHANG Jing WANG Dan ZHANG Jing WANG Yu-hua |
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| Affiliation: | * (College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118) |
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| Abstract: | In this paper, ammonium sulfate precipitation method was used to obtain the soluble protein from Lactobacillus rhamnosus B10 broth. The different protein components: the peak Ⅰ, peak Ⅱ and peak Ⅲ. It were purified and isolated using dextran gel Sephadex G-100 column. Their scavenging capacity of DPPH·, superoxide radicals (O2-·) and hydroxyl radical (·OH) were analysed. The results showed that the soluble protein, peakⅠ, peak Ⅱ and peak Ⅲ has scavenging effect on DPPH·, and the peakⅠ has more DPPH· scavenging capacity than peakⅡ, Ⅲ, the soluble protein is less than peak Ⅱ, Ⅲ. The O2-· and ·OH scavenging capacity of the peak Ⅰ is the strongest, and it was higher than 5 mmol/L Vc solution. The O2-· and ·OH scavenging capacity of the soluble protein is weak. |
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| Keywords: | Lactobacillus rhamnosus soluble protein antioxidant activity |
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