| 固定化碱性蛋白酶水解花生蛋白制备ACE抑制肽 |
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| 引用本文： | 芦鑫,孙强,宋国辉,张丽霞,李婧,黄纪念.固定化碱性蛋白酶水解花生蛋白制备ACE抑制肽[J].食品科技,2012(7):166-171. |
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| 作者姓名： | 芦鑫 孙强 宋国辉 张丽霞 李婧 黄纪念 |
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| 作者单位： | 河南省农科院农副产品加工所;河南省农产品生物活性物质工程技术研究中心 |
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| 基金项目： | 河南省省院科技合作项目(102106000035) |
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| 摘 要： | 采用固定化碱性蛋白酶水解花生蛋白制备ACE抑制肽。以短肽生成率和ACE抑制率作为评价指标,通过响应面优化设计获得最佳的酶解条件为:加酶量1767μ/g蛋白,酶解时间143min,酶解pH为10.00,酶解温度取45℃,获得的短肽生成率和ACE抑制率为82.67%和83.48%。此外,适当增加水解度可以提高酶解产物的ACE抑制活性,但过度水解会导致ACE抑制率下降。
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| 关 键 词： | 降血压肽 花生蛋白 固定化 |
Preparation of antihypertensive peptide from hydrolyzing peanut protein by immobilized alkaline protease |
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| LU Xin,SUN Qiang,SONG Guo-hui,ZHANG Li-xia,LI Jing,HUANG Ji-nian.Preparation of antihypertensive peptide from hydrolyzing peanut protein by immobilized alkaline protease[J].Food Science and Technology,2012(7):166-171. |
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| Authors: | LU Xin SUN Qiang SONG Guo-hui ZHANG Li-xia LI Jing HUANG Ji-nian |
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| Affiliation: | 1,2*(1.Institute of Agricultural Processing,Hennan Academy of Agricultural Sciences,Zhengzhou 450002;2.Henan Engineering Research Centre of Bioactive Substances in Agricultural Products,Zhengzhou 450002) |
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| Abstract: | Immobilized alkaline protease was used to prepare antihypertensive peptide from hydrolyzing peanut protein.With yield rate of peptide and Angiotensin I-Converting Enzyme(ACE) inhibition rate as evaluation indexes,response surface methodology was used to optimize the hydrolytic conditions,which were:enzyme concentration 1767 μ/g protein,hydrolysis time 143 min,pH 10.00,hydrolysis temperature 45 ℃.Under this conditions yield rate of peptide and ACE inhibition rate were 82.67% and 83.48%.Moreover,ACE inhibition increased by heightening degree of hydrolysis(DH),but excessive hydrolysis led to decrease in ACE inhibition. |
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| Keywords: | antihypertensive peptide peanut protein immobilization |
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