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| 1株产细菌素植物乳杆菌的筛选及所产细菌素的理化性质分析 | |
| 引用本文： | 刘丽,郝彦玲,张红星,谢英,周绪宝. 1株产细菌素植物乳杆菌的筛选及所产细菌素的理化性质分析[J]. 中国食品学报, 2011, 11(6) |
| 作者姓名： | 刘丽 郝彦玲 张红星 谢英 周绪宝 |
| 作者单位： | 1. 北京农学院食品科学系,北京,102206 2. 中国农业大学食品科学与营养工程学院,北京,100083 3. 北京市农业绿色食品办公室,北京,100029 |
| 基金项目： | 北京市教委项目; 北京市农委项目 |
| 摘 要： | 从浙江省生产的一种火腿制品中分离筛选到1株具有抑菌活性的乳酸菌菌株LH-09,对单核细胞增生李斯特菌ATCC54003的生长具有良好的抑制作用.16SrRNA序列同源性分析鉴定乳酸菌LH-09为植物乳杆菌.在排除有机酸、过氧化氢的干扰后,确定该抑菌物质为蛋白类物质,即细菌素.理化性质分析表明细菌素LH-09具有较好的热稳定性、酸碱稳定性,可被人体内蛋白酶降解.N-羟甲基甲基甘氨酸-SDS-PAGE电泳确定细菌素LH-09的分子质量在2～6 kDa之间. |
| 关 键 词： | 细菌素 乳酸菌 理化性质 |
Study on Screening of Lactobacillus Plantarum for Producing Bacteriocin and its Physicochemical Characteristics |
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| Liu Li,Hao Yanling,Zhang Hongxing,Xie Ying,Zhou Xubao. Study on Screening of Lactobacillus Plantarum for Producing Bacteriocin and its Physicochemical Characteristics[J]. Journal of Chinese Institute of Food Science and Technology, 2011, 11(6) | |
| Authors: | Liu Li Hao Yanling Zhang Hongxing Xie Ying Zhou Xubao |
| Affiliation: | Liu Li1 Hao Yanling2 Zhang Hongxing1* Xie Ying2 Zhou Xubao3(1College of Food Science,Beijing Agriculture College,Beijing 1022062College of Food Science and Nutritional Engineering,China Agriculture University,Beijing 1000833Beijing Agriculture Greenfood Offices,Beijing 100029) |
| Abstract: | Strain LH-09,isolated from ham products in Zhejiang province,produced antimicrobial substance which was inhibitory to Listeria monocytogenes 54003.Strain LH-09 was identified as Lactobacillus plantarum by using 16S rRNA gene sequence homology analysis.After eliminating the effect of organic acid and hydrogen peroxide,the antimicrobial substance with its proteinaceous nature was confirmed as bacteriocin.Bacteriocin LH-09 was heat-stable and remained activity after incubation at a wide pH.It could be degraded... |
| Keywords: | bacteriocin lactobacillus plantarum physicochmical characteristics |
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