Improved objective fluorodensitometric determination of aflatoxins in cottonseed products

Modifications in the extraction solvent, lead acetate, and silica gel extract purification steps, and the TLC development conditions of the aqueous acetone procedure of Pons et al. [JAOAC49, 554–562 (1966)] for the estimation of aflatoxins in cottonseed products, provides an improved method with essentially quantitative recovery of aflatoxins added to typical cottonseed materials. Both the accuracy and precision of aflatoxin estimates are significantly improved by the incorporation of an objective fluorodensitometric measurement of aflatoxins on TLC plates. Presented at Joint AOCS-AACC Meeting, Washington, D.C., March 31–April 4, 1968. So. Utiliz. Res. Dev. Div., ARS, USDA.