6种食用菌子实体水提物对肠道菌群的影响

以金针菇、香菇、黑木耳、平菇、双孢菇、杏鲍菇6种常见的市售食用菌子实体为研究对象,利用Illumina Mi Seq PE250二代测序平台对肠道微生物V4区进行富集测序,分析6种食用菌子实体水提物对粪便悬液中肠道菌群的菌群种类和菌群结构的影响。测序结果显示经过不同实验组培养后,不同种类的食用菌子实体对肠道微生物的影响各不相同,金针菇组的厚壁菌门水平较高;香菇组的拟杆菌门、变形菌门、厚壁菌门、双歧杆菌和乳杆菌水平较高;黑木耳组的拟杆菌门、放线菌门、厚壁菌门、双歧杆菌和乳杆菌水平较高;平菇组的拟杆菌门、放线菌门、厚壁菌门和乳杆菌水平较高;双孢菇组的拟杆菌门、放线菌门水平较高;杏鲍菇组的放线菌门水平较高。

Effects of Water Extracts from Six Different Species of Edible Mushroom on Intestinal Microorganisms in Humans

This study researched the effects of water extracts from six different species of edible mushroom, Flammulina velutipes, Lentinus edodes, Auricularia auricula, Pleurotus ostreatus, Agaricus bisporus and Pleurotus eryngii, on the microbial community composition and structure in the human intestine. Towards this goal, MiSeq PE250 Illumina nextgeneration sequencing platform was used to identify the sequence of the 16S rDNA V4 region from the bacterial cells. Sequencing results showed that different species of edible mushrooms had different impacts on the intestinal mictrobiota. Higher populations of Firmicutes were found in the Flammulina velutipes group, Bacteroidetes, Proteobacteria, Firmicutes, Bifidobacterium and Lactobacillus in the Lentinus edodes group, Bacteroidetes, Actinomycetes, Firmicutes, Bifidobacterium and Lactobacillus in the Auricularia auricular group, Bacteroidetes, Actinomycetes, Firmicutes and Lactobacillus in the Pleurotus ostreatus group, Bacteroidetes and Actinomycetes in the Agaricus bisporus group, and Actinomycetes in the Pleurotus eryngii group.