响应面试验优化杏鲍菇液体发酵菌种培养条件

通过Box-Behnken响应面试验设计方法对杏鲍菇液体发酵菌种培养条件进行优化。通过单因素试验得出最佳p H值、接种量、转速、装液量。在此基础上通过响应面分析得到了四因素对菌丝干质量得率的影响,其顺序依次为转速p H值接种量装液量。同时得到杏鲍菇液体发酵菌种的最佳培养条件,其具体参数为p H 5.90、接种量9.50%、转速147.00 r/min、装液量95.00 m L/250 m L,且在该条件下杏鲍菇菌丝干质量可到达1.265 7 g/100 m L。本研究结果为杏鲍菇液体菌种栽培技术和活性物质研究提供理论依据。

Optimization of Culture Conditions for Liquid-State Fermentation of Pleurotus eryngii by Response Surface Methodology

In this work, a three-level Box-Behnken factorial design was employed to optimize the culture conditions for liquid-state fermentation of Pleurotus eryngii. By the combined use of one-factor-at-a-time method and response surface methodology, the effect of four different factors on dry mycelial biomass was determined to be in the order of rotation speed > initial pH > inoculum quantity > medium volume and their optimal levels were 147.00 r/min, 5.90, 9.50% and 95.00 mL/250 mL, respectively. Under these conditions, the dry mycelial biomass was 1.265 7 g/100 mL. In conclusion, this study will provide evidence for the cultivation and bioactive components analysis of P. eryngii.