The effect of the N-linked glycans on structural features and physicochemical functions of soybean β-conglycinin homotrimers |
| |
Authors: | Nobuyuki Maruyama Mohamad Ramlan Mohamed Salleh Koji Takahashi Kazuhiro Yagasaki Hideyuki Goto Naho Hontani Shuko Nakagawa Shigeru Utsumi |
| |
Affiliation: | (1) Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, 611-0011 Uji, Kyoto, Japan;(2) National Institute of Crop Science, National Agricultural Research Organization, 305-8518 Tsukuba, Ibaraki, Japan;(3) Nagano Chushin Agricultural Experiment Station, 399-6461 Shiojiri, Nagano, Japan;(4) Department of Food Science, Ishikawa Agricultural College, 921-8836 Nonoichi, Ishikawa, Japan |
| |
Abstract: | β-Conglycinin is a trimeric protein consisting of three subunits, α,α′,and β, which are N-glycosylated. The α and α′ subunits
contain extension regions in addition to core regions common to all subunits. We purified homogeneous trimers consisting of
only α, α′, or β from mutant soybean cultivars containing β-conglycinin lacking one or two subunits: α homotrimers from an
α′-lacking mutant, α′ homotrimers from an α-lacking mutant, and β homotrimers from an α-and α′-lacking mutant. Structural
features and physicochemical functions of the three homotrimers were examined and compared with those of recombinant homotrimers
having no N-linked glycans. The native homotrimers have secondary structures very similar to those of the recombinant ones.
In analogy with the recombinant homotrimers, the native ones exhibit different thermal stabilities from one another (β>α′>α),
and the native α and α′ homotrimers exhibit better solubility, emulsifying ability, and heat-induced association than the
native β homotrimer. Further, the N-linked glycans contribute to solubilities of the three subunits at low ionic strength
(μ=0.08) and to the emulsifying ability of the native β homotrimer. N-Linked glycans also prevent heat-induced associations
of the native α and α′ homotrimers but do not contribute to the secondary structure and the thermal stability of β-conglycinin. |
| |
Keywords: | β -Conglycinin N-linked glycan physiochemical function soybean structural feature |
本文献已被 SpringerLink 等数据库收录! |
|