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On the accuracy of protein determination in large biological samples by prompt gamma neutron activation analysis |
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| Authors: | K. Kasviki I.E. Stamatelatos E. Yannakopoulou J. Kalef-Ezra |
| Affiliation: | a Institute of Nuclear Technology and Radiation Protection, NCSR ‘Demokritos’, Aghia Paraskevi, Attikis 15310, Greece b Medical Physics Laboratory, Medical School, University of Ioannina, Ioannina 45110, Greece c Institute of Physical Chemistry, NCSR ‘Demokritos’, Aghia Paraskevi, Attikis 15310, Greece d Institute of Technology of Agricultural Products, NAGREF, Lycovrissi, Attikis 14123, Greece |
| Abstract: | A prompt gamma neutron activation analysis (PGNAA) facility has been developed for the determination of nitrogen and thus total protein in large volume biological samples or the whole body of small animals. In the present work, the accuracy of nitrogen determination by PGNAA in phantoms of known composition as well as in four raw ground meat samples of about 1 kg mass was examined. Dumas combustion and Kjeldahl techniques were also used for the assessment of nitrogen concentration in the meat samples. No statistically significant differences were found between the concentrations assessed by the three techniques. The results of this work demonstrate the applicability of PGNAA for the assessment of total protein in biological samples of 0.25-1.5 kg mass, such as a meat sample or the body of small animal even in vivo with an equivalent radiation dose of about 40 mSv. |
| Keywords: | 82.80.Jp |
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