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Organic phase PPO biosensor based on hydrophilic films of electropolymerized polypyrrole |
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| Authors: | Cecilia Cristea Serge Cosnier Ionel Catalin Popescu |
| Affiliation: | a University of Medicine and Pharmacy “Iuliu Hatieganu” Cluj-Napoca, Faculty of Pharmacy, Analytical Chemistry Department, RO-400010 Cluj-Napoca, Romania b Laboratoire d’Electrochimie Organique et de Photochimie Redox, UMR CNRS 5630, Institut de Chimie Moléculaire de Grenoble, FR CNRS 2607, Université Joseph Fourier, Grenoble 1, 301 rue de la Chimie, BP 53 Grenoble, Cedex 938041, France c University “Babes-Bolyai” Cluj-Napoca, Department of Physical-Chemistry, RO-400028 Cluj-Napoca, Romania |
| Abstract: | We described herein, the construction of an organic phase enzyme electrode (OPEE) via polyphenol oxidase (PPO) entrapment within a hydrophilic polypyrrole film electrogenerated from on a new bispyrrolic derivative (1) containing a long hydrophilic spacer. The so-called “adsorption step procedure” was adopted for the preparation of the organic phase PPO biosensor. The amperometric detection of catechol was carried out in anhydrous chloroform at −0.2 V versus Ag/AgCl. The electroanalytical parameters of the biosensor strongly depend on its configuration and on the hydration state of the enzyme matrix. The best sensitivity obtained for catechol in chloroform was 15.6 mA M−1 cm−2. |
| Keywords: | Catechol biosensor Polyphenol oxidase Hydrophilic polypyrrole film |
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