| 重组大肠杆菌可溶表达并快速定量GFP-hepA融合蛋白 |
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| 引用本文： | 陈银,邢新会,叶逢春,况莹.重组大肠杆菌可溶表达并快速定量GFP-hepA融合蛋白[J].中国化学工程学报,2007,15(1):122-126. |
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| 作者姓名： | 陈银 邢新会 叶逢春 况莹 |
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| 作者单位： | Department of Chemical Engineering, Tsinghua University, Beijing 100084, China |
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| 摘 要： | To establish a rapid quantification method for heparinase I during its production in recombinant Escherichia coli, a translational fusion vector was constructed by fusing the N terminus of heparinase I to the C terminus of a green fluorescent protein mutant （GFPmutl）. As a result, not only was the functional recombinant expression of heparinase I in E. coli accomplished, but also a linear correlation was obtained between the GFP fluorescence intensity and heparinase I activity, allowing enzyme activity to be quantified rapidly during the fermentation.
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| 关 键 词： | heparinase Ⅰ |
| 收稿时间： | 21 November 2005 |
| 修稿时间： | 2005-11-212006-06-12 |
Soluble Expression and Rapid Quantification of GFP-hepA Fusion Protein in Recombinant Escherichia coli |
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| CHEN Yin, XING Xinhui, YE Fengchun,KUANG Ying.Soluble Expression and Rapid Quantification of GFP-hepA Fusion Protein in Recombinant Escherichia coli[J].Chinese Journal of Chemical Engineering,2007,15(1):122-126. |
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| Authors: | CHEN Yin XING Xinhui YE Fengchun KUANG Ying |
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| Affiliation: | Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;Department of Chemical Engineering, Tsinghua University, Beijing 100084, China |
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| Abstract: | To establish a rapid quantification method for heparinase Ⅰ dtring its production in recombinant Escherichia coli, a translational fusion vector was constructed by fusing the N terminus of heparinase Ⅰ to the C terminus of a green fluorescent protein mutant (GFPmut1). As a result, not only was the functional recombinant expression of heparinase Ⅰ in E. coli accomplished, but also a linear correlation was obtained between the GFP fluorescence intensity and heparinase Ⅰ activity, allowing enzyme activity to be quantified rapidly during the fermentation. |
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| Keywords: | functional expression fusion protein green fluorescent protein (GFP) rapid quantification |
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