| Production of Indigo by Immobilization of E. coli BL21 （DE3） Cells in Calcium-Alginate Gel Capsules |
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| 引用本文： | 陆燕,梅乐和.Production of Indigo by Immobilization of E. coli BL21 （DE3） Cells in Calcium-Alginate Gel Capsules[J].中国化学工程学报,2007,15(3):387-390. |
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| 作者姓名： | 陆燕 梅乐和 |
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| 作者单位： | Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China |
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| 摘 要： | The ability of catalyzing indole into indigo of gene engineering strain expressing P450 BM3 immobilized by entrapment in calcium-alginate gel capsules was examined,and various characteristics of immobilized cells were assessed.Optimum conditions for cells activity were not affected after immobilization,and pH and tempera- ture for both free and immobilized cells were found to be pH 7.5 and 35℃,respectively.The immobilized cells exhibited a markedly improved thermal stability than free cells.After five repeated experiments,the yield of indigo with the immobilized cells retained over 94%of their original activity,which indicated that the operational stability for recycling in batch processes was improved.
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| 关 键 词： | 海澡酸钙胶囊 重组 生产方法 靛蓝 |
| 收稿时间： | 20 June 2006 |
| 修稿时间： | 2006-06-202006-11-07 |
Production of indigo by immobilization of E. coli BL21 (DE3) cells in calcium-alginate gel capsules |
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| LU Yan,MEI Lehe.Production of indigo by immobilization of E. coli BL21 (DE3) cells in calcium-alginate gel capsules[J].Chinese Journal of Chemical Engineering,2007,15(3):387-390. |
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| Authors: | LU Yan MEI Lehe |
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| Affiliation: | Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China |
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| Abstract: | The ability of catalyzing indole into indigo of gene engineering strain expressing P450 BM3 immobilized by entrapment in calcium-alginate gel capsules was examined, and various characteristics of immobilized cells were assessed.Optimum conditions for cells activity were not affected after immobilization, and pH and temperature for both free and immobilized cells were found to be pH 7.5 and 35℃, respectively.The immobilized cells exhibited a markedly improved thermal stability than free cells.After five repeated experiments, the yield of indigo with the immobilized cells retained over 94% of their original activity, which indicated that the operational stability for recycling in batch processes was improved. |
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| Keywords: | calcium-alginate gel gene engineering strain immobilized cells indigo P450 BM3 |
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