| 白藜芦醇对大鼠脑缺血再灌注氧化应激损伤的影响 |
| |
| 引用本文： | 任俊伟,杨琴. 白藜芦醇对大鼠脑缺血再灌注氧化应激损伤的影响[J]. 中国生物制品学杂志, 2011, 24(3) |
| |
| 作者姓名： | 任俊伟 杨琴 |
| |
| 作者单位： | 重庆医科大学附属第一医院神经内科眼科学重庆市市级重点实验室; |
| |
| 基金项目： | 国家自然科学基金(81071119); 重庆市卫生局科研基金(2009-2-359) |
| |
| 摘 要： | 目的探讨白藜芦醇(Resveratrol,Res)对大鼠脑缺血再灌注氧化应激损伤的影响。方法将SD大鼠随机分为假手术组(S组)、缺血再灌注组(I/R组,线栓法复制大鼠右侧大脑中动脉栓塞模型)、Res低剂量组(15 mg/kg,I/R+R1组)和Res高剂量组(30 mg/kg,I/R+R2组),于缺血2 h再灌注24 h进行神经功能缺损评分;化学比色法测定大鼠血清和脑组织中丙二醛(Malondialdehyde,MDA)含量及超氧化物歧化酶(Superoxide dismutase,SOD)活性;TTC法测定脑梗死体积;干湿重法测定脑含水量,HE染色观察脑组织的病理改变。结果与I/R组相比,Res能改善大鼠脑缺血再灌注损伤后的神经功能缺失(P<0.01),降低血清及脑组织中MDA的含量(P<0.01),提高SOD活性(P<0.01),缩小脑梗死体积(P<0.01),降低损伤侧脑含水量(P<0.01),改善脑组织的病理变化,且呈剂量依赖性。结论 Res对大鼠局灶性脑缺血再灌注氧化应激损伤具有良好的保护作用,其机制可能与清除自由基,减轻氧化性损伤有关。
|
| 关 键 词： | 白藜芦醇 脑缺血 再灌注损伤 自由基 丙二醛 超氧化物歧化酶 |
Effect of Resveratrol on Focal Cerebral Ischemia Reperfusion Injury Via Ameliorating Oxidative Stress in Rats |
| |
| REN Jun-wei,YANG Qin. Effect of Resveratrol on Focal Cerebral Ischemia Reperfusion Injury Via Ameliorating Oxidative Stress in Rats[J]. Chinese Journal of Bilogicals, 2011, 24(3) |
| |
| Authors: | REN Jun-wei YANG Qin |
| |
| Affiliation: | REN Jun-wei,YANG Qin(Department of Neurology,The First Affiliated Hospital of Chongqing Medical University,Chongqing Key Laboratory of Ophthalmology,Chongqing 400016,China) |
| |
| Abstract: | Objective To investigate the effect of resveratrol(Res) on focal cerebral ischemia reperfusion injury via ameliorating oxidative stress in rats.Methods SD rats were divided into sham operation(S),ischemia / reperfusion [I / R,i.e.rat model of cerebral artery occlusion(MCAO) copied by intraluminal thread] as well as Res at low(I / R + R1,15 mg / kg) and high(I / R + R2,30 mg / kg) dosage groups,of which the lesions of neurological function were scored 2 h after ischemia and 24 h after reperfusion separately,... |
| |
| Keywords: | Resveratrol(Res) Cerebral ischemia Reperfusion injury Free radicals Malondialdehyde(MDA) Superoxide dismutase(SOD) |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
