The Utilities of Chemical Reactions and Molecular Tools for O‐GlcNAc Proteomic Studies

The post‐translational modification of nuclear and cytoplasmic proteins with O‐linked β‐N‐acetylglucosamine (O‐GlcNAc) is involved in a wide variety of cellular processes and is associated with the pathological progression of chronic diseases. Considering its emerging biological significance, systematic identification, site mapping, and quantification of O‐GlcNAc proteins are essential and have led to the development of several approaches for O‐GlcNAc protein profiling. This minireview mainly focuses on the various useful chemical reactions and molecular tools with detailed reaction mechanisms widely adopted for O‐GlcNAc protein/peptide enrichment and its quantification for comprehensive O‐GlcNAc protein profiling.