一株高产2-苯乙醇酵母菌的筛选及鉴定

从24株不同来源的酵母菌中分离筛选出一株对2-苯乙醇耐受性强、生物转化合成2-苯乙醇能力高的优良菌株SH003,该菌株能在含有4 g/L的2-苯乙醇培养基中生长,在优化的转化条件下,转化合成2-苯乙醇质量浓度达4.31 g/L,生成速率为0.18 g/(L·h),L-苯丙氨酸摩尔转化率为72.4%,经菌落特征、菌体形态分析、生理生化试验,结合18S r DNA序列分析,由系统发育树表明它与酿酒酵母亲缘关系最近,确定该菌株为Saccharomyces cerevisiae。

Screening and Identification of High-yield Strain for 2-Phenylethanol

Abstract A strain designated as SH003 with high 2-phenylethanol tolerance and bioconversion properties was selected from 24 yeast strains with different sources. It could grow in the medium containing 4g/L 2-phenylethanol. Under the optimized conditions, the production and productivity of 2-phenylethanol respectively reached to 4.31g/L and 0.18g/L/h. The molar conversion rate of L-phenylalanine to 2-phecylethanol was 72.4%. Identified by the morphological and physiological characteristics and its 18S rDNA sequence, strain SH003 was closely related with Saccharomyces cerevisiae on phylogenesis.