| 外源蛋白在大肠杆菌中的可溶性表达策略 |
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| 引用本文： | 朱红裕,李强.外源蛋白在大肠杆菌中的可溶性表达策略[J].过程工程学报,2006,6(1):150-155. |
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| 作者姓名： | 朱红裕 李强 |
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| 作者单位： | 清华大学化学工程系生物化工研究所,北京,100084 |
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| 摘 要： | 长期以来，大肠杆菌一直是表达外源蛋白的首选表达系统. 但由于外源蛋白在表达过程中容易被宿主细胞蛋白酶降解或者形成包涵体，其应用受到了限制. 本文综述了在大肠杆菌中表达可溶外源蛋白的策略和进展，以期提高具有生物活性的外源基因的表达水平.
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| 关 键 词： | 外源蛋白 可溶性表达 大肠杆菌 |
| 文章编号： | 1009-606X（2006）01-0150-06 |
| 收稿时间： | 2005-01-04 |
| 修稿时间： | 2005-02-07 |
Strategies for Expression of Soluble Heterologous Proteins in Escherichia coli |
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| ZHU Hong-yu,LI Qiang.Strategies for Expression of Soluble Heterologous Proteins in Escherichia coli[J].Chinese Journal of Process Engineering,2006,6(1):150-155. |
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| Authors: | ZHU Hong-yu LI Qiang |
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| Affiliation: | Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beoijing 100084, China |
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| Abstract: | Escherichia coli has long been the primary expression system for the synthesis of heterologous proteins, but its utility is limited because many heterologous proteins are either prone to be degraded by the cellular proteases or to accumulate in insoluble form, typically as inclusion bodies. This paper reviews recent advances in the strategies for expression soluble heterologous proteins in Escherichia coli so as to improve the recovery yield of heterologous gene products in a biologically active form. |
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| Keywords: | heterologous protein soluble expression Escherichia coli |
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