| 镭普克的制备及对小鼠H22肝癌的抑制作用 |
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| 引用本文： | 唐志红,秦松,吴少杰,高江涛,林莉萍,丁健. 镭普克的制备及对小鼠H22肝癌的抑制作用[J]. 高技术通讯, 2004, 14(3): 83-86 |
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| 作者姓名： | 唐志红 秦松 吴少杰 高江涛 林莉萍 丁健 |
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| 作者单位： | 中国科学院海洋研究所,青岛,266071;中国科学院研究生院,北京,100039;中国科学院海洋研究所,青岛,266071;中国科学院上海药物研究所,上海,200031 |
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| 基金项目： | 863计划(2001AA620410)和中国科学院创新工程(KZCX3-SW-215)资助项目. |
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| 摘 要： | 报道重组别藻蓝蛋白(rAPC，镭普克)表达菌株的20L高密度发酵、镭普克制备及对小鼠H22肝癌抑制作用的实验结果。采用两步发酵法最终OD600为36．18，比一步发酵法的40．16值低，但镭普克表达率是后者的1．8倍，为30．24％，镭普克生物量提高1．62倍。亲合层析结合分子筛层析纯化后，镭普克纯度可达98．03％。对小鼠H22肝癌静脉注射剂量为50mg/kg／d，抑瘤率为62．2％。初步的免疫实验结果表明，镭普克对淋巴B细胞功能具有增强作用。结果提示镭普克大规模生产的潜力和临床应用的可能性。
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| 关 键 词： | 重组别藻蓝蛋白 重组大肠杆菌 高密度发酵 高效表达 抗肿瘤活性 |
Production and Antitumor Bioactivity of rAPC(Recombinant Allophycocyanin) Expressed in a Recombinant E.coli Strain |
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| Tang Zhihong,Qin Song,Wu Shaojie,Gao Jiangtao,Lin Liping,Ding Jian. Production and Antitumor Bioactivity of rAPC(Recombinant Allophycocyanin) Expressed in a Recombinant E.coli Strain[J]. High Technology Letters, 2004, 14(3): 83-86 |
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| Authors: | Tang Zhihong Qin Song Wu Shaojie Gao Jiangtao Lin Liping Ding Jian |
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| Abstract: | High cell density fermentation of a rAPC producing E. coli strain at 20L scale, purification and antitumor effects of rAPC were studied. One step and two step fermentations were employed and the result showed that both expression efficiency and biomass of rAPC in two step fermentation was much higher than those in one step fermentation. After affinity and gel filtration chromatography, the purity of rAPC reached 98.03%. Inhibitory rate of rAPC on H 22 hepatomae could reach 62.2% at the dose of 50 mg/kg per day by i.v application. It was also revealed by experiments that rAPC could inhance the function of B cells by i.p. Result of this paper suggests a promising prospect of large scale production of rAPC and potential clinical application. |
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| Keywords: | Recombinant allophycocyanin Recombinant E. coli High cell density culture High expression Antitumor activity |
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