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Estimation of individual feature surface area with the vertical spatial grid
Authors:L. M. CRUZ-ORIVE  C. V. HOWARD
Abstract:The area of an individual bounded surface (e.g. the boundary of a properly sampled cell) can be estimated from an isotropic uniform random stack of parallel sections, or of non-invasive planar scans, using the well-known spatial grid. A standing problem was to estimate the area of an individual bounded surface with an arbitrary degree of accuracy from a vertical (i.e. not isotropic) stack of sections or scans. A new tool to do this, called the ‘vertical spatial grid’, is presented.
Keywords:Confocal microscopy  cycloids  scanning planes  stereology  surface area  vertical sections  vertical spatial grid
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