螺旋藻多糖对环磷酰胺所致小鼠骨髓抑制的实验研究

目的 研究螺旋藻多糖对小鼠骨髓造血功能的增强作用。方法 将小鼠分成4 组:对照组、环磷酰胺模型组、螺旋藻多糖30 mg·kg^-1、螺旋藻多糖60mg·kg^-1。模型组、螺旋藻多糖组腹腔注射环磷酰胺造模。同时螺旋藻多糖组灌胃螺旋藻多糖, 对照组、模型组灌胃生理盐水。结果 螺旋藻多糖在30、60mg·kg^-1剂量下, 对环磷酰胺所致小鼠外周血象、骨髓有核细胞均有改善(P <0.01) 。对骨髓细胞微核增加有明显的拮抗作用(P <0.05) 。螺旋藻多糖组小鼠骨髓细胞染色体畸变率较模型组显著降低,其抑制率分别为51.2%、61.5 %。结论 螺旋藻多糖可抑制环磷酰胺诱发的小鼠造血功能损伤。

Polysaccharide of spirulina on the prophylaxis and treatment of myelosup- pression of mice caused by cyclophosphamide

Aim The effect of polysaccharide of spirulina on mouse myelosuppression induced by cyclophosphamide was studied. Methods Mice in the model, treatment groups I and H were given cyclophosphamide (ip 100 mg·kg^-1) for there days. At the same time mice in the treatment groups I and II were given ig spirulin 30 mg·kg^-1 and 60 mg·kg^-1 for ten days, while mice in the control group and model group were given ip 20 mg·kg^-1 of NS. Peripheral blood and marrow cytometry, micromcelsus and chromosome aberration were analyed. Results In the dosages of 30 and 60 mg·kg^-1 polysaccharide of spirulina the polysaccharide of spirulina had perfect action on the perihem ateikon and the function of bone hematoplast induced by cyclophosphamide (P＜0.01) and antagonistic action on increase of bone cell micro nuclsus(P ＜0.05); The ratio of chromosome aberration in the treated group was lower compared with that in the model group. The inhibitory ratio was 51.2% and 62.5% in the treated groups I and II. Conclusion Polysaccharide of spirulina can perfect the hematoplast function of mice injured by cyclophosphamide.