Analysis of High Mass-to-Charge Ions in a Quadrupole Ion Trap Mass Spectrometer via an End-Cap Quadrupolar Direct Current Downscan

A method for performing mass-selective instability analysis in a three-dimensional (3-D) quadrupole ion trap is described that involves scanning a direct current (dc) voltage applied to the end-cap electrodes while holding the radio frequency (rf) potential at a fixed value. Rather than eject at the ?(z) = 1 instability line by ramping the amplitude of the drive rf potential applied to the ring electrode, as with the original mass-selective instability scan, this approach effects ion ejection along the ?(z) = 0 instability line in a process identical in principle (though it varies in its method of implementation) to the previously termed "downscan" ( Todd , J. F. J. ; Penman , A. D. ; Smith , R. D. Int. J. Mass Spectrom. Ion Processes 1991 , 106 , 117 - 135 ). A linear scan of the dc amplitude results in a nonlinear mass scale, unlike the conventional resonance ejection scan with a linear scan of the rf amplitude, and the ejection of ions in the direction of high mass-to-charge (m/z) to low m/z. However, the downscan offers some advantages over the traditional rf scan for ions of high m/z values. These include a larger scannable mass range, as well as the opportunity for improved resolution at high mass. These characteristics are demonstrated with ions of m/z 10(4)-10(5).