| 食源性单增李斯特菌的ERIC-PCR和Sau-PCR分型研究 |
| |
| 引用本文： | 赵一鸣,石磊,孟赫诚,张志刚,闫鹤.食源性单增李斯特菌的ERIC-PCR和Sau-PCR分型研究[J].食品与机械,2015,31(1):54-58. |
| |
| 作者姓名： | 赵一鸣 石磊 孟赫诚 张志刚 闫鹤 |
| |
| 作者单位： | 华南理工大学轻工与食品学院，广东 广州 510640；福建省厦门银祥集团有限公司，福建 厦门 361100 |
| |
| 基金项目： | 国家自然科学基金青年科学基金项目（编号：31201363）；广东省自然科学基金(编号：10451064101005159) |
| |
| 摘 要： | 对从广州市天河区超市和菜市场及厦门某食品加工厂分离得到的单增李斯特菌进行ERIC-PCR和Sau-PCR检测,进行溯源研究及遗传多样性分析。ERIC-PCR将22株单增李斯特菌共分为8个基因型,其中以h型为主,共有8株菌。Sau-PCR方法将这批菌分为7个基因型,主要型别为E型和G型,二者共占50%。两种分型方法的结果呈现出一定的差异与关联,使用两种不同分型方法进行综合分析,有助于更好地认识单核细胞增生李斯特菌(LM)菌株间的遗传关系和流行病学特点。
|
| 关 键 词： | 单增李斯特菌 ERIC-PCR Sau-PCR 溯源研究 遗传多样性 |
| 收稿时间： | 2014/9/26 0:00:00 |
ERIC-PCR and Sau-PCR analysis of food-borne Listeria monocytogenes |
| |
| ZHAOYiming,SHILei,MENGHecheng,ZHANGZhigang,YANHe.ERIC-PCR and Sau-PCR analysis of food-borne Listeria monocytogenes[J].Food and Machinery,2015,31(1):54-58. |
| |
| Authors: | ZHAOYiming SHILei MENGHecheng ZHANGZhigang YANHe |
| |
| Affiliation: | College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, Guangdong 510640, China; Xiamen Yinxiang Group CO., Ltd, Xiamen, Fujiang 361100, China |
| |
| Abstract: | To investigate the distribution of serotypes, genetic relativity and epidemiological characteristic of Listeria monocytogenes (LM). 22 LM strains isolated from Guangzhou and Xiamen were investigated by ERIC-PCR and Sau-PCR. Results of ERIC-PCR showed that these strains were distributed in 8 patterns (a-h). The main pattern was pattern h, and the number of strains was 8. Genotyping using Sau-PCR showed that all tested strains were grouped into 7 clusters (A-G). These results indicated the genetic relativity and epidemiological characteristic of different LM food-borne isolates. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《食品与机械》浏览原始摘要信息 |
|
点击此处可从《食品与机械》下载全文 |
|
