胃癌超微结构酶细胞化学研究：五种细胞器标志酶的电镜观察

本文应用超微结构酶细胞化学方法对14例胃癌细胞和1例正常胃粘膜上皮细胞ALPase、ACPase、G6Pase、TPPase和CCOase等五种细胞器标志酶进行了超微结构水平的定位观察,结果表明:1.正常胃粘膜上皮无ALPase反应,ACPase、G6Pase、TP-Pase和CCOase均见于相应细胞器,与其功能代谢相一致。2.部分管状腺癌细胞AL-Pase定位于腔缘及肠型微绒毛,提示它们可能来源于肠化上皮或癌细胞具有向肠上皮分化的倾向;部分管状腺癌细胞ALPase无反应,但保留着胃粘膜上皮的某些特征,表明可能来源于胃固有粘膜上皮。粘液腺癌TPPase和ACPase反应明显,其高尔基体较发达,浣酶体泌噬作用活跃,说明其分泌和消化功能均较强。低分化腺癌各标志酶无反应或反应微弱,与其相应细胞器功能低下或结构异常有关。

Ultrastructural Cytochemistry of Human Gastric Cancer

The distribution of alkaline phosphatase(ALPase), acid phosphatase(ACPase), glucose-6-phosphatase (G6Pase), thiamine pyrophosphatase(TPPase)and cytochrome oxidase(CCO)in 14 cases of gastric cancers and I case of normal gastric epithelium were studied ultrastructurally. The results showed:I. normal gastric epitheli-um had no ALPase reaction except capillary endotheliaI cells. The reactions of ACPase, G6Pase, TPPase and CCO were found in the corresponding organellae, which were cosistent with functions. 2. in tubular adenocarci- noma cells, the reactions of ACPase, G6Pase, TPPase and CCO were more apparent in the corresponding or- ganellae. Some cells of tubular adenocarcinoma showed ALPase reaction products, which lay on the intestine- type microvilli surfaces, indicating that these cells might originate fron intestinal metaplasia or have a tendency to differentiate intestinal epithelium, the others showed no ALPase reaction products, but still retained some characteristics of gastric epithelium, indicating these cells might originate from gastric mucosa; The mucinous adenocarcinoma cells showed higher ACPase and TPPase reactions objectively, indicating that these cells had stronger ability of digestion and secretion; In poorly differentiated adenocarcinoma cells, ALPase, ACPase, G6Pase TPPase and CCO showed negative or faint reaction products, indicating that their corresponding or- ganellae were immature or disorganized.