| 表面等离子体共振成像检测桃胶多糖与半乳糖凝集素-3的相互作用 |
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| 引用本文： | 帅玉环,齐攀,李莹,胡翠英,蔡梦洁,冉艳红,李仕萍,钟金钢.表面等离子体共振成像检测桃胶多糖与半乳糖凝集素-3的相互作用[J].激光与光电子学进展,2019,56(9):230-237. |
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| 作者姓名： | 帅玉环 齐攀 李莹 胡翠英 蔡梦洁 冉艳红 李仕萍 钟金钢 |
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| 作者单位： | 暨南大学理工学院物理学系,广东广州,510632;广东交通职业技术学院电子工程系,广东广州,510650;暨南大学预科部,广东广州,510610;暨南大学生命科学技术学院生物工程学系,广东广州,510632;暨南大学理工学院光电工程系,广东广州,510632 |
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| 基金项目： | 国家自然科学基金;广东高校省级重点平台;重大科研项目;广东省自然科学基金;广东省高等职业院校珠江学者岗位计划 |
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| 摘 要： | 利用自组装的数字全息表面等离子体共振成像技术,分别检测了两种具有不同分子质量的桃胶多糖(PGP-1与PGP-2)与半乳糖凝集素-3的相互作用。制备了表面等离子体共振成像生物芯片,同时检测了具有不同浓度的桃胶多糖样品与半乳糖凝集素-3的结合过程,制作了标准曲线,并计算了相互作用的结合平衡常数。结果表明,两种具有不同分子质量的桃胶多糖可以直接结合半乳糖凝集素-3,其中PGP-1的结合平衡常数为8.36×10^5M^-,PGP-2的结合平衡常数为1.24×10^5M^-。结合曲线符合生物分子相互作用的规律,证明了该方法在多通量生物检测中的可行性。该方法实验装置简单、易操作、无需标记、成本低,在高通量分析技术中具有一定的应用前景。
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| 关 键 词： | 表面光学 表面等离子体共振成像 数字全息 桃胶多糖 半乳糖凝集素-3 分子间相互作用 高通量 |
Detection of Interaction Between Peach-Gum Polysaccharides and Galectin-3 via Surface Plasmon Resonance Imaging |
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| Shuai Yuhuan,Qi Pan,Li Ying,Hu Cuiying,Cai Mengjie,Ran Yanhong,Li Shiping,Zhong Jingang.Detection of Interaction Between Peach-Gum Polysaccharides and Galectin-3 via Surface Plasmon Resonance Imaging[J].Laser & Optoelectronics Progress,2019,56(9):230-237. |
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| Authors: | Shuai Yuhuan Qi Pan Li Ying Hu Cuiying Cai Mengjie Ran Yanhong Li Shiping Zhong Jingang |
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| Affiliation: | (Department of Physics, College of Science and Engineering, Jinan University, Guangzhou Guangdong, 510632, China;Department of Electronics Engineering, Guangdong Communication Polytech nic, Guangzhou,Guangdong 510650, China;Pre-University, Jinan University, Guangzhou, Guangdong 510610, China;Department of Bioengineering, College of Life Science and Technology, Jinan University,Guangzhou, Guangdong 510632, China;Department of Optoelectronic Engineering, College of Science and Engineering, Jinan University,Guangzhou, Guangdong 510632, China) |
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| Abstract: | Shuai Yuhuan;Qi Pan;Li Ying;Hu Cuiying;Cai Mengjie;Ran Yanhong;Li Shiping;Zhong Jingang(Department of Physics, College of Science and Engineering, Jinan University, Guangzhou Guangdong, 510632, China;Department of Electronics Engineering, Guangdong Communication Polytech nic, Guangzhou,Guangdong 510650, China;Pre-University, Jinan University, Guangzhou, Guangdong 510610, China;Department of Bioengineering, College of Life Science and Technology, Jinan University,Guangzhou, Guangdong 510632, China;Department of Optoelectronic Engineering, College of Science and Engineering, Jinan University,Guangzhou, Guangdong 510632, China) |
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| Keywords: | optics at surfaces surface plasmon resonance imaging digital holography peach gum polysaccharide galectin-3 biomolecular interaction high throughput |
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