基于结构的丝氨酸蛋白酶超家族进化分析

丝氨酸蛋白酶是一类水解酶,得名于Ser195对底物的亲核攻击.丝氨酸蛋白酶在长期进化过程中,虽然蛋白质序列之间的差异性很大,但仍然保持了一个稳定的核心催化结构 ;同时,丝氨酸蛋白酶也衍生出各种各样的生物学功能.但是,序列的差异以及大量的复制现象使得丝氨酸蛋白酶超家族的进化研究进展缓慢.本文用SSAP算法从结构比较的层次上进行蛋白质超家族进化的研究,分析了丝氨酸蛋白酶超家族结构与功能的演化,研究了丝氨酸蛋白酶结构上的进化及其对功能的影响,对于蛋白质结构与功能关系的研究以及蛋白质分子设计都有一定的指导意义.

Structure Based Evolution Analysis of the Serine Protease Superfamily

The serine protease superfamily is a subgroup of the hydrolases,whose name comes from the conserved Se-r195 cat alyzing the substrates.Serine proteases maintain a common fold over a very long evolutionary span,in spite of divergent sequences.This class of enzymes has developed a wide variety of important biological functions.However,strongly divergent protein sequences and a mass of gene duplications make it very difficult to perform evolution analysis on the whole superfamily.In the present work,structure based evolution analysis was performed on the serine proteases superfamily using SSAP algorithm,and the structure and function evolution were analyzed,especially the effect on functions by structure evolution of the serine proteases.The research would have certain guiding significance on the relationships between protein structures and functions and the molecular design.