Feasibility study on cellular network analysis with patterned cell culture microdevice |
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| Authors: | Takahiro Kawashima Tsuyoshi Kimura Takayuki Shibata Akio Kishida Takashi Mineta Eiji Makino |
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| Affiliation: | 1. Department of Production Systems Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Toyohashi 441-8580, Japan;2. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan;3. Faculty of Science and Technology, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561, Japan;1. Institute of Pharmacology, Heidelberg University, Heidelberg 69120, Germany;2. Institute for Anatomy and Cell Biology, Heidelberg University, Heidelberg 69120, Germany;3. Max-Planck Institute for Heart and Lung Research, Bad Nauheim 61231, Germany;4. Department of Screening and Compound Profiling, Molecular Discovery Research, GlaxoSmithKline, Hertfordshire, UK;1. Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908, USA;2. Division of Infectious Diseases and International Health, University of Virginia Health System, Charlottesville, VA 22908, USA;3. Seattle Structural Genomics Center for Infectious Disease (SSGCID), USA;4. Emerald Bio, Bainbridge Island, WA 98110, USA;5. Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA 22908, USA;6. Department of Medicine, University of Washington, Seattle, WA 98195, USA;7. Seattle Biomedical Research Institute, Seattle, WA 98109, USA;8. Departments of Global Health and Medical Education & Biomedical Informatics, University of Washington, Seattle, WA 98195, USA;9. Department of Medicine, University of Virginia, Charlottesville, VA 22908, USA;10. Department of Pathology, University of Virginia, Charlottesville, VA 22908, USA;1. Department of Pharmacology, University of Tennessee Health Science Center, Memphis, TN, USA;2. Department of Pharmaceutical Sciences, University of Tennessee Health Science Center, Memphis, TN, USA;3. Department of Physiology, University of Tennessee Health Science Center, Memphis, TN, USA;4. Research Service, Veteran’s Affairs Medical Center, Memphis, TN, USA |
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| Abstract: | In order to realize cellular network analysis on a chip-based system, our group has been developing a patterned cell culture microdevice with pillars in an array for tapping cells into space surrounded by the pillars. The pillar structures has advantages to trap both adhesive and non-adhesive cells and to precisely control positions of cells and distances between cells for understanding effects of various cell patterns on functions of a cellular network such as cell proliferation, differentiation, and network formation. In this paper, HeLa cell cultivation with the patterned cell culture microdevice having a pillar array fabricated by dry film of thick negative photoresist SU-8 on a glass substrate was executed as a feasibility study on a cellular network analysis. The results revealed that the device performance was found to be enough to culture HeLa cells for more than 48 h. In addition, relative extensibility of blocks of multiple cells compared with single cells tapped on the device was observed. Thus, the patterned cell culture microdevice proposed here could be applicable to analysis of cellular functions. |
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