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人肺组织内源性显微荧光特性研究
引用本文:郑蔚,黄志伟,S.M.Krishnan,Chia Teck Chee.人肺组织内源性显微荧光特性研究[J].中国激光,2001,28(7):669-672.
作者姓名:郑蔚  黄志伟  S.M.Krishnan  Chia Teck Chee
作者单位:1. 福建师范大学激光研究所;Biomedical Engineering Research Center, Nanyang Technological University,
2. 福建师范大学激光研究所;School of Science, Nanyang Technological University,
3. Biomedical Engineering Research Center, Nanyang Technological University,
4. School of Science, Nanyang Technological University,
基金项目:国家自然科学基金 (编号 :697780 2 9),福建省科委国际科技合作计划资助项目
摘    要:采用新型显微荧光分光光度系统 ,实现激光诱导人肺组织内源性显微荧光光谱与图像的测量 ,获得不同组织层 (即上皮层 ,粘膜层和软骨层 )的荧光分布和荧光特性 ,为探索癌与正常组织自体荧光光谱特征差异的根源 ,揭示激光诱导自体荧光法诊断和定位早期肺癌的机理提供重要的实验依据

关 键 词:显微荧光分光光度系统  激光诱导自体荧光  内源性显微荧光光谱与图像  人肺组织
收稿时间:2000/3/7

Intrinsic Microscopic Fluorescence and Imaging of Human Lung Tissues
ZHENG Wei , HUANG Zhi wei , XIE Shu sen S. M. Krishnan Teck Chee Chia Institute of Laser,Fujian Normal University,Fuzhou Biomedical Engineering Research Centre,Nanyang Technological University,Singa.Intrinsic Microscopic Fluorescence and Imaging of Human Lung Tissues[J].Chinese Journal of Lasers,2001,28(7):669-672.
Authors:ZHENG Wei  HUANG Zhi wei  XIE Shu sen S M Krishnan Teck Chee Chia Institute of Laser  Fujian Normal University  Fuzhou Biomedical Engineering Research Centre  Nanyang Technological University  Singa
Affiliation:ZHENG Wei 1,2 HUANG Zhi wei 1,3 XIE Shu sen 1 S. M. Krishnan 2 Teck Chee Chia 3 1Institute of Laser,Fujian Normal University,Fuzhou 350007 2Biomedical Engineering Research Centre,Nanyang Technological University,Singa
Abstract:The microscopic fluorescence spectra and imaging of excised lung tissue sections were studied under a novel microspectrophotometer system using a He Cd laser light at 442 nm. Differences of the intrinsic autofluorescence distributions in different tissue layers, i.e., epithelium, mucosa and cartilage, were observed in normal and malignant lung tissues. The results show that the study of microscopic fluorescence on various tissue layers can be used to explore the origin of spectral differences between normal and abnormal lung tissues, which is very useful for the better understanding of the mechanism of the early cancer diagnosis using the laser induced autofluorescence technique.
Keywords:microspectrophotometer system  laser  induced autofluorescence  microscopic fluorescence spectroscopy and imaging  human lung tissue
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