金颗粒为活性基底的裸鼠血清表面增强拉曼散射光谱分析

对肿瘤裸鼠血清进行显微共聚焦拉曼光谱测量,并将血清的普通拉曼光谱与以金纳米颗粒为基底的表面增强拉曼散射(SERS)光谱进行对比,结果表明金纳米粒子作为活性基底对裸鼠血清具有显著的拉曼增强作用,在很多常规拉曼光谱中未能检测出的信息在SERS光谱中得以体现。这源于血清中的物质与金纳米颗粒之间的化学吸附和相互作用,使得微量样品也能够通过SERS光谱来反映出血清中各种蛋白质、脂类、糖类、核酸以及其他成分的分子结构等信息。血清中各种成分的信息可以反映体内各种细胞和组织的生理代谢及病理变化,血清的SERS光谱将为疾病的诊断和治疗提供一种有效手段。

Surface Enhanced Raman Scattering Spectrum Analysis of Nude Mouse Serum with Au Nanoparticles Active Substrate

Raman spectrum and surface enhanced Raman scattering (SERS) spectrum of the nude mouse serum are measured using laser Raman confocal micro-spectrometry. The results show that the Au nanoparticles active substrate can enhance the Raman spectra dramatically. A lot of information which conventional Raman spectroscopy fails to detect emerges. This is due to the chemical adsorption and interactions between the gold nanoparticles and the substances in serum. The information is important for the analysis of the most components and structures of the serum proteins. With SERS spectrum, even micro-sample can also reflect the molecular structure information of the serum proteins, lipids, carbohydrates, ribonucleic acid and other components. Information of the serum components can reflect physiological and pathological metabolism changes of the cells and tissues in the body. So SERS spectrum of the serum would provide an effective means for the diagnosis and therapy of diseases.