| 骨髓间充质干细胞在特定微环境中自然分组向成骨细胞和内皮细胞分别分化的可行性研究 |
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| 引用本文： | 王嫣,陈小菊,徐杰,于海生,罗月苹,周兰,陈文直.骨髓间充质干细胞在特定微环境中自然分组向成骨细胞和内皮细胞分别分化的可行性研究[J].四川激光,2011(2):51-53. |
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| 作者姓名： | 王嫣 陈小菊 徐杰 于海生 罗月苹 周兰 陈文直 |
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| 作者单位： | [1]重庆医科大学生物医学工程系 省部共建超声医学工程国家重点实验室 超声医学工程重庆市市级重点实验室,重庆400016 [2]重庆医科大学检验系,重庆400016 [3]重庆医科大学附属第二医院海扶肿瘤治疗中心,重庆400010 |
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| 基金项目： | 国家重点基础研究发展计划-973计划项目（2011CB707902）; 国家自然科学基金青年科学基金项目（31000435）; 重庆市科委自然科学基金项目（CSTC 2009BB5261） |
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| 摘 要： | 目的:观察骨髓间充质于细胞(bone mesenchymal stem cells,BMSCs)在特定的微环境中是否具有自然分组向成骨细胞和内皮细胞(endothelial cell,EC)两种不同细胞同时分化的可能.方法:将BMSCs采用成骨诱导剂和成内皮细胞诱导剂以1:1的比例混合培养12天.倒置显微镜和HE染色观...
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| 关 键 词： | 组织工程 干细胞 分化 |
Study of inducing bone mesenchymal stem cell to form osteoblast and endothelial cell in one microenvironment |
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| WANG Yan,CHEN Xiao-ju,XU Jie,YU Hai-sheng,LUO Yue-ping,ZHOU Lan,CHEN Wen-zhi.Study of inducing bone mesenchymal stem cell to form osteoblast and endothelial cell in one microenvironment[J].Laser Journal,2011(2):51-53. |
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| Authors: | WANG Yan CHEN Xiao-ju XU Jie YU Hai-sheng LUO Yue-ping ZHOU Lan CHEN Wen-zhi |
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| Affiliation: | 1.State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science and Technology,Chongqing Key laboratory of Ultrasound in Medicine and Engineering,Department of Biomedical Engineering,Chongqing Medical University,Chongqing 400016,China;2.Department of Laboratory Medicine,Key Laboratory of Medical Diagnostics,Ministry of Education,Chongqing Medical University,Chongqing 400016,China;3.Clinic Center for Ultrasound Therapy of 2nd Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China) |
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| Abstract: | Objective：To explore the possibility of inducing BMSCs to differentiate into osteoblasts and EC in same microenvironment simultaneously.Method： BMSCs were cultured in the mixing medium which contains dexamethasone,ascorbic acid,β-glycerophosphate,VEGF and L-glutamine for 12 days.Cells were observed by microscope and HE stain,incubated with anti-vWF and anti-Osteocalcin,and analysed the differentiation rates from BMSCs to EC by image analysis.Immunofluorescence with anti-vWF and anti-Osteocalcin were observed by laser scanning confocal microscopy（LSCM）.Results： The different morphous can be observed by light microscope and HE stain： some cells formed calcified nodules and some cells formed a tube similarity.Osteocalcin and vWF were positive in the two kinds of cells;the density of EC was 14.1%.After fluorescence immunocytochemistry staining, two colors can be detected in cells simultaneously with LSCM.The mix of two colors was yellow,but the colors of some cells were much red,some were much green.Conclusion： BMSCs could differentiate into osteoblasts and EC in the same microenvironment when precisely induced. |
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| Keywords: | tissue engineering stem cell differentiation |
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