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人参总皂苷对K562细胞的增殖抑制及诱导分化作用
引用本文:雷翠蓉,姜蓉,王红宁,何轩,左国伟,陈地龙,官涛,王建伟.人参总皂苷对K562细胞的增殖抑制及诱导分化作用[J].四川激光,2011(2):69-70,73.
作者姓名:雷翠蓉  姜蓉  王红宁  何轩  左国伟  陈地龙  官涛  王建伟
作者单位:重庆医科大学附一院,重庆400016
基金项目:国家自然科学基金资助项目(No:30970872)
摘    要:目的:研究人参总皂苷TSPG对人慢性粒细胞白血病细胞株(K562细胞)增殖及分化的影响,探讨TSPG抗肿瘤作用.方法:取对数生长期K562细胞,分为阴性对照组、不同浓度TSPG组及阳性对照组进行体外培养,以噻唑蓝(MTT)比色法、台盼蓝活细胞计数法观察TSPG对K562细胞增殖的影响;用联苯胺染色及血红蛋白测定检测其诱...

关 键 词:人参总皂苷  K562细胞  增殖抑制  分化

Effects of total saponins of panax ginseng on proliferation and differentiation of K562 cells
LEI Cui-rong,JIANG Rong,WANG Hong-ning,HE Xuan,ZUO Guo-wei,CHEN Di-long,GUAN Tao,WANG Jian-wei.Effects of total saponins of panax ginseng on proliferation and differentiation of K562 cells[J].Laser Journal,2011(2):69-70,73.
Authors:LEI Cui-rong  JIANG Rong  WANG Hong-ning  HE Xuan  ZUO Guo-wei  CHEN Di-long  GUAN Tao  WANG Jian-wei
Affiliation:(The First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China)
Abstract:Objective: To investigate the growth inhibition and differentiation of K562 cells induced by total saponins of panax ginseng(TSPG).Method: Different concentrations of TSPG were added to K562 cells at different times.The inhibition effects of TSPG on K562 cells were observed by MTT and trypan blue staining.The differentiation of K562 cells was examined using Benzidine staining and hemoglobin synthesis test.The apoptosis rates of K562 cells induced by TSPG were analyzed by flow cytometry.Result: TSPG have the ability to inhibit the proliferation of K562 cells in the dose-and time-dependent manners.TSPG could increase hemoglobin synthesis(P0.05) but Benzdine-positive K562 cells induced by TSPG were not increased.TSPG at 400mg/L raised apoptosis rate of K562 cells compared with control group(P0.05).Conclusion: TSPG may inhibit the proliferation of K562 cells and induce their apoptosis and differentiation.
Keywords:total saponins of panax ginseng(TSPG)  K562 cells  growth inhibition  differentiation
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