| 血小板衍生生长因子、骨保护素对体外培养成骨细胞的功能影响 |
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| 引用本文： | 张冬根,谭颖徽,裘松波,牙祖蒙,张纲,王建华.血小板衍生生长因子、骨保护素对体外培养成骨细胞的功能影响[J].四川激光,2005,26(3):94-95. |
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| 作者姓名： | 张冬根 谭颖徽 裘松波 牙祖蒙 张纲 王建华 |
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| 作者单位： | 第三军医大学附属新桥医院口腔科,重庆,400037;第三军医大学附属新桥医院口腔科,重庆,400037;第三军医大学附属新桥医院口腔科,重庆,400037;第三军医大学附属新桥医院口腔科,重庆,400037;第三军医大学附属新桥医院口腔科,重庆,400037;第三军医大学附属新桥医院口腔科,重庆,400037 |
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| 基金项目： | 第三军医大学回国人员启动基金资助,编号 :XG2 0 0 3 64 |
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| 摘 要： | 目的:研究血小板衍生生长因子(platelet-derivedgrowthfactor ,PDGF)对成骨细胞(osteoblast,OB)的功能影响及PDGF与骨保护素(os teoprotegerin ,OPG)在成骨细胞中表达的关系。方法:用酶消化和组织块移行生长相结合的方法,从幼兔颅骨中分离出成骨细胞进行培养,应用组织学和酶细胞化学对其生物学特性进行鉴定,兔抗PDGF -BB、OPG抗体诱导培养成骨细胞,分析成骨细胞的增殖、分化、矿化及OPGmRNA表达。结果:PDGF -BB抗体组与对照组在成骨细胞的分化成熟及OPGmRAN表达方面有显著差异(P <0 .0 1)。结论:PDGF -BB抗体可抑制OB的分化成熟、骨质形成及OPGmRAN的表达。
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| 关 键 词： | 成骨细胞 兔抗PDGF-BB、OPG OPG MRNA表达 |
| 文章编号： | 0253-2743(2005)03-0094-02 |
| 修稿时间： | 2004年4月5日 |
Effects of platelet-derived growth factor and osteoprotegerin on functions of osteoblast cell in vitro |
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| ZHANG Dong-gen,TAN Ying-hui,QIU Song-bo,YA Zhu-meng,ZHANG Gang,WANG Jian-hua.Effects of platelet-derived growth factor and osteoprotegerin on functions of osteoblast cell in vitro[J].Laser Journal,2005,26(3):94-95. |
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| Authors: | ZHANG Dong-gen TAN Ying-hui QIU Song-bo YA Zhu-meng ZHANG Gang WANG Jian-hua |
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| Abstract: | Objective:To do research on the influence of osteoblast cells about platelet-derived growth factor and the connection among platelet-derived growth factor and osteoprotegerin on osteoblast cells. Methods :The osteoblast cells used were isolated from the calvariae of neonatal (one-day-old) rabbits by sequential enzymatic digestion and bony tissues.The cells were identified with histology and enzymohistochemical observation, induced by the anti-rabbit antibody of PDGF-BB and OPG, analyzed with osteoblast cells on multiplication, differentiation,mineralization and the expression of OPG mRNA. Results: The anti-rabbit antibody PDGF-BB groups were much significantly lower than that of control groups,this effect depends on the dose of antibody. Conclusions:The osteoblast cells were restrained by the anti-rabbit antibody PDGF-BB on multiplication,maturation, building of bone and expression of OPG mRAN. |
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| Keywords: | Osteoblast Cells Anti-rabbit Antibody of PDGF-BB and OPG Expression of OPG mRNA |
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