姜黄素诱导肺癌A549细胞凋亡

目的：研究姜黄素对A549细胞凋亡的诱导作用，并探讨其可能的分子机制。方法：姜黄素处理A549细胞后，MTT法于不同时间点检测A549细胞的增殖情况；姜黄素处理A549细胞48h后，流式细胞术（Flow CytoMetry，FCM）检测A549细胞的凋亡率；30μmol／L姜黄素处理A549细胞24h、48h、72h后，RT—PCR、Western—Blot检测A549细胞中P53、Bcl2、Bax和cagpase-3基因的表达水平。结果：姜黄素对肺癌A549细胞增殖的抑制作用具有显著的浓度-时间依赖关系，30μmol／L姜黄素可诱导A549细胞凋亡。30μmoL／L姜黄素作用A549细胞24h即可引起A549细胞中P53、Bax和Caspase-3表达水平的上调，48h后可引起Bcl2表达水平的下调，诱导A549细胞凋亡。结论：30μmol／L姜黄素可诱导A549细胞凋亡，其机制可能与促进P53、Bax和Caspase-3基因的表达，抑制Bcl2基因的表达有关。

Curcmnin induced apoptosis in Lung Cancer A549 Cells

Objective：To study the induced effect of curcumin on apoptosis in Lung Cancer 3-549 Cells and explore the possible mechanisms. Methods： The proliferation of A549 cells was detected by MTT assay at different time points after curcumin treatment; the apoptosis of A549 cells was detected by FCM at 48 hours after curcumin treatment. The expression levels of P53, Bcl2, Bax, Caspase - 3 were detected by RT - PCR, Western - Blot at 48 hours after curcumin treatment. Result： Inhibitory effect of curcumin on proliferation in Lung Cancer 3.549 Cells has a significant concentration- time dependent manner. 30μmol/L can induce the apoptosis of A549 cells. The expression levels of P53, Bax and Caspase - 3 on A549 cells were up - regulation at 24 hours after 30μmol / L curcumin treatment; the expression level of Bcl2 on A549 cells was down - regulation at 48 hours after 30μmol/L curcumin treatment; the apoptosis of A549 cells was induced at 48 hours after 30μmol/L curcumin treatment. Conclusion：30μmol/L curcumin can induce the apoptosis of A549 cells, the mechanism may be related to the promotion of P53, Bax and Caspase - 3 gene expression, inhibition of Bcl2 gene expression.