| 血卟啉单甲醚介导的光动力作用对小鼠肝癌细胞生长抑制的初步研究 |
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| 引用本文： | 黄曦,周溯.血卟啉单甲醚介导的光动力作用对小鼠肝癌细胞生长抑制的初步研究[J].四川激光,2010(4):76-77. |
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| 作者姓名： | 黄曦 周溯 |
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| 作者单位： | [1]重庆医科大学生物医学工程系,重庆市生物医学工程学重点实验室、重庆市超声医学_T-程重点实验室一省部共建国家重点实验室培育基地,重庆400016 [2]四川石油管理局总医院,成都610213 |
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| 摘 要： | 目的：观察血卟啉单甲醚介导的光动力作用对小鼠肝癌H22细胞生长的抑制作用。方法：采用倒置显微镜观察和MTT法检测血卟啉单甲醚介导的光动力作用后小鼠肝癌H22细胞的生长情况。结果：血卟啉单甲醚介导的光动力作用显著抑制小鼠肝癌H22细胞的生长,在一定光能密度条件下,细胞生长抑制作用跟血卟啉单甲醚浓度间呈剂量依赖关系。倒置显微镜下观察到光动力作用组癌细胞数量显著减少,细胞固缩,而单纯光照组,单纯血卟啉单甲醚组和假照射组三组间无明显差异;MTT法示光动力组细胞抑制率显著高于单纯光照组、单纯血卟啉单甲醚组和假照射组（P〈0.05）,单纯血卟啉单甲醚组和假照射组间无明显差异（P〉0.05）。结论：血卟啉单甲醚光动力学作用对小鼠肝癌细胞H22生长抑制作用确切。
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| 关 键 词： | 光动力学疗法 肝癌 血卟啉单甲醚 |
Photodynamic inhibition of hematoporphyrin monomethyl ether in hepatoma cells |
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| HUANG Xi,ZHOU Su.Photodynamic inhibition of hematoporphyrin monomethyl ether in hepatoma cells[J].Laser Journal,2010(4):76-77. |
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| Authors: | HUANG Xi ZHOU Su |
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| Affiliation: | 1.Department of Biomedical Engineering in Chongqing Medical University,Institute of Ultrasound Engineering in Medicine of Chongqing University of Medical Sciences, Sate Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and MOST,Chongqing,400016,China;2.CNCP Southwest Oil and Gas Hospital,Chengdu of Sichuan 610213,China) |
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| Abstract: | Objective：To explore photodynamic inhibition of Hematoporphyrin Monomethyl Ether（HMME） in Hepatoma Cells.Methods：The photocytotoxic potency and efficacy of HMME 24h after PDT in hepatoma cell line H22 was investigated by inverted microscope and MTT reduction assay.Results：Inverted microscope and MTT reduction assay showed that the photodynamic inhibition of HMME in H22 was extraordinarily significant.The photocytotoxicity of HMME indicated dose dependent characteristics in H22 cells.Conclusion：HMME induced PDT could effectively kill H22 cells and HMME will become an alternative photosensitizer for photodynamic therapy. |
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| Keywords: | photodynamic therapy hepatoma hematoporphyrin monomethyl ether |
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