| 低强度中频超声对白血病细胞株k562杀伤作用的初步研究 |
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| 引用本文： | 何勇,夏新蜀,余和平,白定群,谭勇,许川山. 低强度中频超声对白血病细胞株k562杀伤作用的初步研究[J]. 激光杂志, 2009, 30(2): 69-70 |
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| 作者姓名： | 何勇 夏新蜀 余和平 白定群 谭勇 许川山 |
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| 作者单位： | 重庆医科大学超声影像学研究所,重庆,400010;重庆医科大学附属第二医院康复理疗中心,重庆,400010;重庆医科大学附属第一医院康复医学科,重庆,400016 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的：研究低强度中频超声对白血病细胞株K562的杀伤作用。方法：取对数生长期的K562细胞，分成实验组和假辐照组，用MTT法和台盼兰染色计算细胞抑制率。结果：低强度中频超声照射各组间细胞抑制率与对照组相比有统计学差异，且组间存在统计学差异。结论：单纯低强度中频超声对白血病细胞株K562有较强的杀伤作用，且其杀伤效应与照射强度和时间明显相关。
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| 关 键 词： | 单纯超声 白血病细胞 杀伤效应 |
Primary study on lethal effect of ultrasound on human malignant myeloid cell lines K562 |
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| HE Yong,,XIA Xin-shu,YU He-ping,BAI Ding-qun,TAN Yong,XU Chuan-shan. Primary study on lethal effect of ultrasound on human malignant myeloid cell lines K562[J]. Laser Journal, 2009, 30(2): 69-70 |
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| Authors: | HE Yong XIA Xin-shu YU He-ping BAI Ding-qun TAN Yong XU Chuan-shan |
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| Affiliation: | HE Yong1,3,XIA Xin-shu2,YU He-ping2,BAI Ding-qun2,TAN Yong2,XU Chuan-shan1,3 1.Institute of Ultrasound Imaging,Chongqing Medical University,Chongqing 400010,China,2.Department of Rehabilitation Medicine,First Affiliated Hospital,Chongqing 400016,3.Center of Rehabilitation Medical,Second Affiliated Hospital |
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| Abstract: | Objective:To investigate the lethal effect of ultrasound on leukemia cell lines K562.Methods:K562 cells in log phase were divided into sham radiation group and experimental group.The intensity of ultrasound(1.7 MHz) was 1.35 and 2.90W.cm-2 respectively.The exposure time were 5,10,15,20 and 25s respectively.K562 cells were incubated in 24 well culture plates for 24 hours after sonication and the number of vital cells was tested by MTT assay and Trypan blue staining.Results:The absorbance value of MTT and the... |
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| Keywords: | ultrasound malignant myeloid cells lethal effect |
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