| 卡氏肺孢子菌肺炎鼠肺模式识别受体Dectin-1、TLR-2和TLR-4的表达变化 |
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| 引用本文： | 郑玉强,刘爱波,蒲瑜,蔡辉,叶彬.卡氏肺孢子菌肺炎鼠肺模式识别受体Dectin-1、TLR-2和TLR-4的表达变化[J].四川激光,2013(6):110-112. |
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| 作者姓名： | 郑玉强 刘爱波 蒲瑜 蔡辉 叶彬 |
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| 作者单位： | [1]重庆医科大学附属儿童医院临检中心 儿童发育疾病研究教育部重点实验室儿科学重庆市重点实验室重庆市儿童发育重大疾病诊治与预防国际科技合作基地,重庆400014 [2]重庆医科大学病原生物教研室,重庆400016 |
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| 基金项目： | 中国国家自然科学青年基金（81000745）;重庆市卫生局医学科学项目（2009）66,（2010）51. |
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| 摘 要： | 目的：研究卡氏肺孢子菌（Pnemnocystis carinii，Pc）肺炎鼠肺Dectin-1、TLR-2和TLR-4的表达，探讨模式识别受体表达改变与PCP疾病发生的相互关系。方法实验分4组：正常对照组、Pc刺激组、PCP模型组以及PCP模型恢复组。地塞米松磷酸钠免疫抑制方法建立PCP动物模型，改良四胺银（GMS）染色检测Pc包囊；肺组织切片HE染色观察肺组织病理变化；实时荧光定量和Western—blot检测Dectin-1、TLR-2和TLR-4的mRNA以及蛋白的表达。结果：Pc刺激组的Decdn-1、TLR-2以及TLR-4的mRNA以及蛋白的表达明显高于正常对照组（P〈0．05）；Pc刺激组和PCP恢复组Dectin-1的mRNA以及蛋白显著高于PCP组（P〈0．05），而肺部Pc载荷量显著低于PCP组（P〈0．05）。结论地塞米松抑制了PCP鼠肺Dectin-1、TLR-2和TLR-4受体的表达，这可能与PCP疾病的发生和发展有关。
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| 关 键 词： | 卡氏肺孢子菌 Dectin-1 TLR-2 TLR-4 大鼠模型 |
Expression of Dectin - 1, TLR - 2 and TLR - 4 in Pneumocystis carinii pneumonia rat |
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| ZHENG Yu - qiang,LIU Ai - bo,PU Yu,CAI Hui,YE Bin.Expression of Dectin - 1, TLR - 2 and TLR - 4 in Pneumocystis carinii pneumonia rat[J].Laser Journal,2013(6):110-112. |
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| Authors: | ZHENG Yu - qiang LIU Ai - bo PU Yu CAI Hui YE Bin |
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| Affiliation: | 1 .Laboratory Medicine, Childrens Hospital of Chongqing University of Medical Sciences,Ministry of Education Key Laboratory of Child Development,Key Laboraory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperration Center for Child Development and Disorders,Chongqing 400014,China; 2. Department of Pathogen Biology, Chongqing University of Medical Sciences, Chongqing 400016, China) |
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| Abstract: | Objective： To study the expression of Dectin - 1 ,TLR - 2 and TLR-4-in Pneumocystis carinii pneumonia （PCP） rat, and to explore the influence of dexamethasone on the expression of Dectin - 1 ,TLR- 2 and TLR - 4 and its relationship with progression of PCP. Methods Rat were divided into four groups which were normal control, treated with P. carinii {Pc）, PCP model and recovered PCP model. PCP rat were induced with dexamethasone in this study, and Pc cysts were detected by GMS stains. Real - time PCR and Western blot were utilized to detected the expression of Dectin - 1, TLR - 2 and TLR - 4 in lung. Results The expression of Dectin- 1,TLR- 2 and TLR-4 were much higher in the treated group with Pc than that of the normal group（ P 〈0.05}. The expression of Dectin - 1 were much higher in the treated group and recovered PCP group than that of PCP model group{P 〈 0.05）, while the burden of Pccysts was just the reverse（P〈 0.05）. Conclusion Dexamethasone inhibited the expression of Dectin- 1 ,TLR- 2 and TLR-4 in lung of rat , which may be one of mechanisms of PCP development induced by P. carinii. Key words： Pneumocvstis carinii ; dectin - 1, TLR - 2; TLR - 4 ; rat modle |
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| Keywords: | Pneumocvstis carinii dectin - 1 TLR - 2 TLR - 4 rat modle |
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