| LAMS对胃癌细胞化疗的增敏作用 |
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| 引用本文： | 吴晓健,董蒲江.LAMS对胃癌细胞化疗的增敏作用[J].四川激光,2008,29(4). |
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| 作者姓名： | 吴晓健 董蒲江 |
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| 作者单位： | 重庆医科大学第一附属医院普外科 |
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| 摘 要： | 目的:探讨LAMS对胃癌细胞化疗的增敏作用。方法:RT-PCR及免疫组化法测定LAMS作用胃癌细胞前后Bcl-2基因蛋白含量;MTT法分别测定5-Fu、MTX、MMC、EPI、CTX与LAMS联合及单独作用胃癌细胞的ID50以及有效作用时间。结果:LAMS使胃癌细胞Bcl-2 mR-NA及基因蛋白表达下降,使胃癌细胞对5-Fu、MTX、MMC、EPI、CTX的敏感度增加、有效作用时间延长。结论:LAMS可有效地增加胃癌细胞对5-Fu、MTX、MMC、EPI、CTX的敏感性。
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| 关 键 词： | 昆布多糖硫酸酯 胃癌 化疗 |
Sensitization of LAMS on the' chemotherapy for human gastric cancer cells |
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| WU Xiao-jian,Dong Pu-jiang.Sensitization of LAMS on the'''' chemotherapy for human gastric cancer cells[J].Laser Journal,2008,29(4). |
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| Authors: | WU Xiao-jian Dong Pu-jiang |
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| Abstract: | Objective:To study the sensitization effect of laminarin sulphate(LAMS) on the chemotherapy for gastric cancer cells. Methods:RT-PCR and Immunohistochemical stainings were used to determine the change of Bcl-2 protein treated with LAMS.MTT assay was used to examine the ID50 and the effective time of gastric cancer cells treated with 5-Fu,MTX,MMC,EPI,CTX or combined with LAMS.Results:The expression of Bcl-2 mRNA and protein decreased in the gastric cancer cells treated with LAMS.The sensitivity of human gastric cancer cells to 5-Fu,MTX,MMC,EPI,CTX increased and their effective time were extended.Conclusion:The sensitivity of human gastric cancer cells to 5-Fu,MTX,MMC,EPI,CTX can be enhanced by LAMS. |
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| Keywords: | LAMS gastric cancer chemotherapy |
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