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原生动物扫描电镜样品制备方法的探讨
引用本文:顾福康,倪兵.原生动物扫描电镜样品制备方法的探讨[J].电子显微学报,1993,12(6):525-529.
作者姓名:顾福康  倪兵
作者单位:华东师范大学生物系 上海200062
摘    要:以腹毛目纤毛虫棘尾虫、尖毛虫和游仆虫,膜口目纤毛虫草履虫以及腰鞭目鞭毛目角藻为材料,对原生动物扫描电镜样品制备方法作了探讨,提出了样品制备过程中取材固定、脱水、干燥后原生动物在样品台上的定位等值得注意的问题,取得成功的关键及一些体会。

关 键 词:原生动物样品  SEM  生物显微技术

The Exploration of Preparing Protozoan Specimens for Scanning Electron Microscopy
Gu Fukang,Ni Bing.The Exploration of Preparing Protozoan Specimens for Scanning Electron Microscopy[J].Journal of Chinese Electron Microscopy Society,1993,12(6):525-529.
Authors:Gu Fukang  Ni Bing
Affiliation:Gu Fukang,Ni Bing Department of Biology,East China Normal University,Shanghai 200062
Abstract:The methods of preparing protozoan specimens for scanning electron microscopy are suggested here,tested on Stylonychia mytilus,Oxytricha platystoma,Euplotes encysticus and Euplotes sp.(Ciliophora,Hypotrichida), Paramecium caudatum and Paramecium bursaria Ciliophora,Hymenostomatida)and Ceratium hirundinella (Mastigophora,Dinoflagellida).The detailed procedures(collecting of the cells,fixative and fixation,dehy- drating,positioning of the dried cells on the specimen stub)are introduced.Several problems to be noticed are forwarded,and some experences for obtaining good results are also given.
Keywords:Protozoan specimen  Preparing  Scanning electron microscopy
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