郎格罕氏组织细胞增生症的电镜研究

目的：探讨郎格罕氏组织细胞增生症（LCH）的超微病理特点，提供Birbeck颗粒来源的线索。方法：结合光镜、免疫组化及临床资料。对2例LCH进行了超微结构观察。结果：朗格罕组织细胞胞浆内可见棒状、网球拍状Birbeck颗粒，这些细胞免疫组化CDla阳性。结论：LCH的诊断须见到典型的郎格罕氏细胞（Langerhans cell，LC）LC细胞，电镜下找到Birbeck颗粒，或CDla（010）阳性。Birbeck颗粒可与细胞膜相连续，细胞膜凹陷可在胞质内形成Birbeck颗粒。

Electron microscopic studies on Langerhans-cell histiocytosis

Objective:To study ultrastructural features of Langerhans-cell histiocytosis(LCH)and provide some ultrastructural evidence to clarify the origin of Birbeck granules.Method:2 cases of LCH were selected randomly.Specimens were treated with routine LM and TEM procedures,immunohistochemical method,and examined by light and electron microscope.Results:The Langerhans' cells showed positive immunostain for CD1a and S-100 protein.Under the electron microscope,ultrastructure of the Langerhans' cells revealed typical intracytoplasmic tennnis racquet-shaped structure with a central zipper-like striation(Birbeck's granules).Conclusion:Birbeck's granules can serve as a useful marker for the identification of Langerhans-cell histiocytosis.The CD1a and S-100 protein are demonstrated by immunohistochemical method,can serve this purpose as well.The development of Birbeck's Langerhans-cell granules is in evidence by invagination of the cell membrane ultrastructually.