|
|||||
|
|
| AFM的DNA样品制备技术研究 | |
| 引用本文： | 蔡明辉,赵葵,展永,倪嵋楠,隋丽,孔福全,杨明建.AFM的DNA样品制备技术研究[J].电子显微学报,2006,25(1):76-79. |
| 作者姓名： | 蔡明辉 赵葵 展永 倪嵋楠 隋丽 孔福全 杨明建 |
| 作者单位： | 1. 中国原子能科学研究院,北京,102413;河北工业大学理学院,天津,300130 2. 中国原子能科学研究院,北京,102413;北京师范大学射线束技术与材料改性教育部重点实验室,北京,100875 3. 河北工业大学理学院,天津,300130 4. 中国原子能科学研究院,北京,102413 |
| 摘 要： | AFM应用中最为关键的一步无疑是样品的制备。本文介绍DNA样品制备的几种主要方法，通过实验发展了两种适合对DNA及其碎片进行长度测量和做统计分析的制样方法，它们分别采用APS-云母和纯云母为衬底。这两种方法不仅丰富了DNA样品制备方法，对推广AFM在生物研究中的应用也具有重要意义。 |
| 关 键 词： | 原子力显微镜 质粒DNA 样品制备 APS-云母 云母 |
| 文章编号： | 1000-6281（2006）01-0076-04 |
| 收稿时间： | 2005-08-29 |
| 修稿时间： | 2005-08-292005-10-24 |
Study of the DNA sample for atomic force microscopy |
|
| CAI Ming-hui,ZHAO Kui,ZHAN Yong,NI Mei-nan,SUI Li,KONG Fu-quan,YANG Ming-jian.Study of the DNA sample for atomic force microscopy[J].Journal of Chinese Electron Microscopy Society,2006,25(1):76-79. | |
| Authors: | CAI Ming-hui ZHAO Kui ZHAN Yong NI Mei-nan SUI Li KONG Fu-quan YANG Ming-jian |
| Affiliation: | 1 China Institute of Atomic Energy, Beijing 102413 ;2 School of Science, Hebei University of Technology,Tianjin 300130; 3 The Key Laboratory of Beam Technology and Modification of Ministry of Education, Beijing Normal University, Beijing 100875, China |
| Abstract: | The key point of AFM application is the preparation of the sample. Several methods of preparing DNA sample were introduced in this paper. Two methods were developed experimentally for the length measurement of DNA and the fragments induced by radiation, as well as statistical analysis. The APS-mica and bare mica were used as substrates respectively. |
| Keywords: | atomic force microscopy plasmid DNA sample preparation APS- mica mica |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |