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盼生安对人肝癌细胞超微结构的影响
引用本文:李煜,陈德蕙,杨素霞,周淑珍,刘国廉.盼生安对人肝癌细胞超微结构的影响[J].电子显微学报,2004,23(3):201-204.
作者姓名:李煜  陈德蕙  杨素霞  周淑珍  刘国廉
作者单位:1. 北京市疾病预防控制中心,北京,100013
2. 北京基础医学研究所,北京,100850
3. 解放军总医院泌尿外科,北京,100853
4. 北京市博莱特医药技术有限公司,北京,100850
摘    要:目的:以氟尿嘧啶(5-Fu)作为阳性对照观察了盼生安对BEL-7402肝癌细胞超微结构的影响,为该药物的抑癌作用提供形态学依据。方法:用透射电镜观察经不同剂量药物处理后细胞超微结构的变化。结果:经盼生安处理后,胞浆空泡化,细胞器中线粒体严重空泡变性,其次是高尔基体和粗面内质网的空泡化,核糖体减少。胞内次级溶酶体,多泡小体,自噬体及髓样小体增多。5-Fu对肝癌细胞形态的影响不如盼生安明显。结论:盼生安主要导致显著的线粒体超微结构改变,胞浆空泡化,抑制了肿瘤细胞的能量代谢和蛋白质合成系统而使细胞功能减退,代谢缓慢最终使细胞退变死亡。

关 键 词:盼生安  肝癌细胞  超微结构  形态学
文章编号:1000-6281(2004)03-0201-04

Ultrastructural morphological changes of human hepatoma cells after exposure to Panshengan
LI Yu,CHEN De-hui,YANG Su-xia,ZHOU Shu-zhen,LIU Guo-lian.Ultrastructural morphological changes of human hepatoma cells after exposure to Panshengan[J].Journal of Chinese Electron Microscopy Society,2004,23(3):201-204.
Authors:LI Yu  CHEN De-hui  YANG Su-xia  ZHOU Shu-zhen  LIU Guo-lian
Affiliation:LI Yu~1,CHEN De-hui~2,YANG Su-xia~3,ZHOU Shu-zhen~4,LIU Guo-lian~4
Abstract:The ultrastructural morphological changes of BEL-7402 hepatoma cells after exposure to Panshengan were observed by electron microscopy using Fluorouracil as positive control in order to provide evidence for its anticancer effect. Cultured hepatoma cells after exposure to different doses of the drug showed that vesicles and vacuoles were formed in cytoplasma of hepatoma cells. The mitochondria were markedly vesiculated. The vesiculation of Golgi apparatus and rough endoplasmic reticulum were also observed but less than those in the mitochondria. In addition, free ribosomes decreased apparently, while secondary lysosomes, multivesicular bodies and autophagosomes increased. The effect of Flurouracil on hepatoma cells was not as apparent as Panshengan. The results indicate that Panshengan can cause remarkable ultrastructural change of mitochondria and eventually suppress the energy metabolism and protein synthesis in the cells and result in the death of hepatoma cells.
Keywords:Panshengan  morphology  ultrastructure
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