Dual‐Stage‐Light‐Guided Tumor Inhibition by Mitochondria‐Targeted Photodynamic Therapy |
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| Authors: | Kai Han Qi Lei Shi‐Bo Wang Jing‐Jing Hu Wen‐Xiu Qiu Jing‐Yi Zhu Wei‐Na Yin Xu Luo Xian‐Zheng Zhang |
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| Affiliation: | 1. Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan, P.R. China;2. The Institute for Advanced Studies, Wuhan University, Wuhan, P.R. China;3. Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan, P.R. China |
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| Abstract: | In this paper, a self‐delivery system PpIX‐PEG‐(KLAKLAK)2 (designated as PPK) is fabricated to realize mitochondria‐targeted photodynamic tumor therapy. It is found that the PPK self‐delivery system exhibited high drug loading efficacy as well as novel capacity in generation of intracellular reactive oxygen species (ROS). This study also indicated that the photochemical internalization effect of the photosensitizer protoporphyrin IX (PpIX) under a short time light irradiation improved the cellular internalization of PPK. On the contrary, PPK could target to the subcellular organelle mitochondria due to the presence of proapoptosis (KLAKLAK)2 peptide. Importantly, the in situ generation of ROS in mitochondria enhanced the photodynamic therapy efficacy under another long time irradiation, leading to significant cell death with decreased mitochondrial membrane potential. Besides, relative high tumor accumulation, minimal systemic cytotoxicity and efficacious long‐term tumor inhibition in vivo are also confirmed by using a murine model. All these results demonstrated the self‐delivery system PPK with a dual‐stage light irradiation strategy is a promising nanoplatform for tumor treatment. |
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| Keywords: | mitochondria‐target photochemical internalization photodynamic therapy proapoptosis peptide synergistic therapy |
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