| 氧化亚铁硫杆菌培养条件的优化 |
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| 引用本文： | 肖雷,姚菁华,陶秀祥,苏显中,刘爱国.氧化亚铁硫杆菌培养条件的优化[J].选煤技术,2008(6). |
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| 作者姓名： | 肖雷 姚菁华 陶秀祥 苏显中 刘爱国 |
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| 作者单位： | 1. 中国矿业大学化工学院,江苏,徐州,210008
2. 徐州市城北垃圾综合处理有限公司,江苏,徐州,210000 |
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| 基金项目： | 国家自然科学基金，江苏徐州市社会发展项目 |
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| 摘 要： | 氧化亚铁硫杆菌生长代谢影响因素有很多,本试验主要对温度、pH、培养基的量以及接种量进行研究,同时也研究了不同氮源及能源条件对菌生长的影响,确定出氧化亚铁硫杆菌的最佳培养条件为温度28℃,pH=2.0,装液量为100mL(250mL锥形瓶),接种量为10%,最容易利用的能源物质是FeSO4,氮源是硫酸铵。
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| 关 键 词： | 氧化亚铁硫杆菌 培养条件 生长代谢 影响 |
Influence Factors on Growth and Metabolism of Thiobacillus Ferrooxidans |
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| Xiao Lei et al.Influence Factors on Growth and Metabolism of Thiobacillus Ferrooxidans[J].Coal Preparation Technology,2008(6). |
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| Authors: | Xiao Lei |
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| Affiliation: | Xiao Lei et al |
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| Abstract: | There are many factors that influence the growth activities of Thiobacillus ferrooxidans (T.f), and the temperature, initial pH, the quantity of the culture medium and inoculation amount were researched. The results show that the T.f has the highest activity under pH=2.0 and at the temperature of 28℃,100ml in 250ml flat-bottom flasks, and the quantity of inoculation 10%.The utilization of nitrogen and energy sources by Thiobacillus ferrooxidans was studied. The results showed that among 2 kinds of selected energy sources, the ferrous salts were easy to be used by T.f and ammonium sulfate is better nitrogen. |
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| Keywords: | Thiobacillus ferrooxidans ferrous iron optimization |
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